Werkmodul Fachmodul

Microscopy.jpg

Lecturer: Julian Chollet
Credits: 6 ECTS, 4 SWS
Date: Monday (15:15 – 16:45) + individual trainings
Venue: Monday-meeting online / trainings in Marienstraße 7b, DIY BioLab (Room 202)



Students

Submitted works

Denise - Echoes
http://denisenicolau.it/index.html

Sascha - Looks
File:Looks_Sascha_Mirtschin_Microscopy_1st_Semester_Julien_Chollet.pdf

Anna - Spores
https://cloud.uni-weimar.de/s/46XRnyKLwBsG9cf?path=%2FAnna%20Strietzel

Sisu - Dusting dusting
https://cloud.uni-weimar.de/s/46XRnyKLwBsG9cf?path=%2FMon%20Sisu%20Satrawaha



Introduction videos

Great introduction to light microscopy (not only) for kids:
https://www.youtube.com/watch?v=tVcEEw6qbBQ

Glossary

by Denise Nicolau

  • magnification: making images appear larger
  • resolution: ability to distinguish between two objects
  • slide: flat rectangles of thin glass which hold the sample under the microscope
  • sample: something you collect to examine
  • specimen: the sample on the slide when you look at it through the microscope
  • cover slip/cover glass: a smaller and thinner sheet of glass that is placed over the specimen
  • light microscope: it uses light in order to see an image
  • electron microscope: it uses electron beams to see images (you can see a virus)
  • compound microscope: it has two sets of lenses (eyepiece lenses - objective lenses)
  • objective lenses: a set of lenses with different magnifications (e.g., 4x, 10x, 40x - 4 times: lowest magnification, 40 times: highest magnification)
  • total magnification: is given from the multiplication between eyepiece lens magnification and objective lens magnification (e.g., 10x ocular and 4x objective > 40x total magnification).
  • coarse focus and fine focus: two knobs which raise or lower the stage which helps with focusing.
  • stage knobs: the move from side to side which helps exploring/scanning the specimen.
  • mounting: securing the sample on the slide
  • dry mount: a simple kind of mounting where the object is merely placed on the slide (this mounting can be successfully used for viewing specimens like pollen, feathers, hairs, etc.)
  • wet mount: the specimen is placed in a drop of water or other liquid held between the slide and the cover slip. (this method is commonly used to view microscopic organisms that grow in pond water or other liquid media).
  • disposable pipette
  • stage clips: clips which secure the slide
  • lens paper: the only paper you can use to wipe lenses
  • staining: a technique used to enhance contrast in samples
  • immersion oil: able to increase resolution (applicable if you have a 100x objective lens)
  • fixation: it refers to the process of attaching cells to a slide; it is often achieved either by heating or chemically treating the specimen. It kills microorganisms in the specimen, stopping their movement and metabolism while preserving the integrity of their cellular components for observation
  • simple staining: a single dye is used to emphasize particular structures in the specimen
  • differential staining: more dyes are used to emphasize particular structures in the specimen

Syllabus

Our air, soil and water as well as all plants and animals contain complex ecosystems that are colonized by various creatures - most of which we can only see and interact with by virtue of microscopy or cultivation techniques.

This course aims to introduce you to the methodologies, project planning and experimental strategies in the natural sciences – mainly through the use of microscopy techniques. In line with the concept of a hybrid semester, the theoretical input will be communicated through online lectures while the practical skills will be acquired in individualized block-modules. While working in the DIY BioLab (Chair of Media Environments) you will learn how to use different kinds of microscopes and start your own little research project. Beyond the microscopy itself, educational objectives include literature research, experiment design, result documentation and scientific writing.

The course will be structured in a flexible way, tailored to the needs of the participants. Attendance during the online-seminars and hands-on module, as well as the delivery of detailed project documentation (paper, artwork, etc.) until the end of the semester is required. The course can be combined with the module “Flagelates, Nematodes, and I” by Mindaugas Gapševičius.

Basic concepts

Life

"There are over a 100 definitions for 'life' and all are wrong"
http://www.bbc.com/earth/story/20170101-there-are-over-100-definitions-for-life-and-all-are-wrong

https://en.wikipedia.org/wiki/Life#Definitions
Since there is no unequivocal definition of life, most current definitions in biology are descriptive. Life is considered a characteristic of something that preserves, furthers or reinforces its existence in the given environment. This characteristic exhibits all or most of the following traits:

   Homeostasis 
   Organization
   Metabolism
   Growth
   Adaptation
   Response to stimuli
   Reproduction

Organism

https://en.wikipedia.org/wiki/Organism
In biology, an organism (from Greek: ὀργανισμός, organismos) is any individual entity that exhibits the properties of life. It is a synonym for "life form". Organisms are classified by taxonomy into specified groups such as the multicellular animals, plants, and fungi; or unicellular microorganisms such as a protists, bacteria, and archaea. All types of organisms are capable of reproduction, growth and development, maintenance, and some degree of response to stimuli.

Prokaryote

Prokaryotes are represented by two separate domains—bacteria and archaea. A unicellular organism that lacks a membrane-bound nucleus, mitochondria, or any other membrane-bound organelle.

Eukaryote

Eukaryotic organisms are characterized by the presence of a membrane-bound cell nucleus and contain additional membrane-bound compartments called organelles (such as mitochondria in animals and plants and plastids in plants and algae, all generally considered to be derived from endosymbiotic bacteria). Fungi, animals and plants are examples of kingdoms of organisms within the eukaryotes.

Microorganism

Living beings that are too small to be seen with the (human) eye.

https://en.wikipedia.org/wiki/Microorganism
https://en.wikipedia.org/wiki/Human_interactions_with_microbes

They live in almost every habitat from the poles to the equator, deserts, geysers, rocks and the deep sea. Some are adapted to extremes such as very hot or very cold conditions, others to high pressure or [...] high radiation environments. Microorganisms also make up the microbiota found in and on all multicellular organisms.

Microbes are important in human culture and health in many ways, serving to ferment foods, treat sewage, produce fuel, enzymes and other bioactive compounds. They are essential tools in biology as model organisms and have been put to use in biological warfare and bioterrorism. They are a vital component of fertile soils. In the human body microorganisms make up the human microbiota including the essential gut flora. They are the pathogens responsible for many infectious diseases and as such are the target of hygiene measures.

The possible existence of unseen microbial life was suspected from ancient times, such as in Jain scriptures from 6th century BC India and the 1st century BC book On Agriculture by Marcus Terentius Varro. Microbiology, the scientific study of microorganisms, began with their observation under the microscope in the 1670s by Antonie van Leeuwenhoek.


Suggested resources regarding the proposed projects/topics

Cytoplasmic streaming

PDFs

File:Guide_to_scientific_writing.pdf


Links