GMU:Flagelates, Nematodes, and I/Brogen Berwick

From Medien Wiki

Project

Growth and Discovery

Through development we grow. Through discovery we grow.

We start as an infant and we travel our way through life building and growing as we go. We discover new landscapes and environments, and we navigate our way through the challenges, and we overcome these obstacles. We adapt to different spaces and we continue to grow. What are we? One may think we are the species of humankind. We share many behavioural characteristics with humankind, but we are not. We are Physarum polycephalum a species of slime mould. And to grow we discover, and to discover we grow. Through this methodology we succeed, and you can join us through this process of discovery.

As I discover I advance

Documentation

This Wiki page explores my weekly process, the layout shows my week by week process that the viewer can read and look through in order to understand my development .


My project this semester started with a fascination towards Nematodes and their movement abilities. Through exploring this research I discovered that Nematodes eat each other and I became fascinated whether they would eat their offspring. While following this research direction I tried to cultivate Nematodes so that I could begin to work and experiment with them hands on and physically. Unfortunately this was not such a success and I found myself disappointment when I was finally able to see Nematodes under the microscope. I had anticipated that the movements of the Nematode would be much more thrilling and exciting, but this was not the case, I was left rather under-whelmed.

But at this moment of dissatisfaction I meet SLIME MOULD and was completely overwhelmed and excited by this very visual moving and growing cell. As a result I started to research and explore slime mould both with hands on experiments and book research.

Now my project is currently exploring the relationship between slime mould with the introduction of colour pigments both to the slime moulds food source and to its environment.

Week commencing 02/11/2020

Initial Microscope research - Week one

Research about Nematodes

  • Cannibal worms recognise their children—and don’t eat them as a result

YouTube video by Science Magazine Youtube

  • Meet the Most Important Animal You’ve Never Seen

Youtube video by SciShow channel Youtube

Week commencing 09/11/2020

Documentary style videos

Week commencing 16/11/2020

Movements

Nematode vs human??

Can you human body performance like a Nematode? Can the human body move like a Nematode? Can the human body become like a Nematode? Can a human move like a Nematode, is it physically possible?

SOMA - Dance Performers

As the human body is at the heart of SOMA, our dictionary of the physical movement within the performance is defined by its abilities and inabilities. We ask ourselves what the human body is capable of expressing and what are its limitations.

Nematode Movements

Examples that show the way in which Nematodes move and position themselves

Week commencing 23/11/2020

Netflix - My Octopus Teacher

Notes from watching the documentary

  • There is something to learn here, something special.
  • He totally mapped the octopuses environment to learn from her
  • There was total trust between the octopus and him
  • He almost became the octopus, he was obsessed
  • The octopus taught him to become sensitised to the other
  • Our lives were mirroring each others
  • The octopus sacrifices her own life for her young
  • In my mind I think like an octopus
  • That is there strategy - live fast die young
  • The octopus showed me just how precious the wild places are

Week commencing 30/11/2020

First tests with the phone microscope

Screenshot 2020-11-20 at 15.58.37.png

Week commencing 01/12/2020

Making a medium for the Nematode

I found a DIY recipe to make an agar plate to hopefully awaken the Nematodes

Making my own Growth Medium

Ingredients for the recipe

  • 0.5g Peptone
  • 0.3g Yeast
  • 1.5g Agar
  • 0.5g Salt
  • 120ml Water

Screenshot 2020-11-20 at 16.13.28.png

Reflections

Sadly, I don't think that my medium recipe contained enough Agar because the solution didn't solidify, it remained a kind of liquid solution.

How to improve the recipe - add more agar to the recipe?

2nd Attempt to make Medium

IMG 7331.JPG IMG 7332.JPG

Reflections

Because the previous recipe didn't solidify, for this recipe I added 0.5g more Agar to the mixture and then heated up the solution and poured it back into the petri dishes to then store in the fridge again. Hopefully this time the solution will solidify.

Can you see the Nematodes?

Screenshot 2020-11-23 at 17.08.29.png

Are the Nematodes alive in the agar I created? Are they swimming or sleeping?

Reflections

After cooling the Agar in the fridge I waited 1 hour before emptying my Nematodes into the 5 different petri-dishes. I checked on them on an hourly basis to see if they were waking up and moving...Sadly I don't think they are alive. There was no movement. After 2 days the agar seemed to separate, it became very liquidy and a kind of kind grew over the top of the Agar. I don't think my agar recipe worked

After a few days my medium solution had separated, I don't think I created the correct ratio of the ingredients

Sixpetidish.jpg

Moving nematodes - pocket microscope

After sourcing some more Nematodes I used the pocket microscope to see if I could see them moving, and yes they were moving, however it was really difficult to keep the pocket microscope still so I plan to vie them under the microscope in the lab so that I have more control.

To view the videos larger I have uploaded them to YouTube

Week commencing 14/12/2021

WOW I found out about slime mould

Youtube video by the BBC about the importance of slime mould in our woodlands Youtube

Screenshot 2020-12-09 at 12.08.41.png

  • Relentless shape shifting goo
  • It eats anything that gets in it's path
  • Essential service in cleaning up our woodlands
  • White fungus is it's pray
  • When all food is gone the slime mould co-operates with itself to mass produce
  • It's not a plant, animal or fungus, just one giant cell
  • Moves 1cm an hour
  • The whole thing is a network
  • Slime mould can learn about it's surroundings

Trying to revive some dried slime mould

I will now wait 1 hour in the hope that the slime mould is becoming hydrated and will eat the oats!

After 3 days the slime mould didn't revive itself, so I will source some more slime mould!

Week commencing 21/12/2020

Cultivating slime mould

Looking under the Microscope

Using both the phone extension microscope, the pocket microscope and the Lab microscope

Week commencing 28/12/2020

Crossing borders with slime mould

Over the Christmas break me and my slime mould went on a journey back to the UK

Slimemould.JPG

Here you can see I tried to revive my slime mould, I bought Petri dishes and Agar to make the right environment for the slime mould but as you can see the slime mould didn't grow.


Mouldy.JPG

I wanted to wait a few more days to see if the slime mould would be able to grow, therefore I gave each Petri dish some more oat flakes to entice the slime mould to feed but it was unsuccessful and the more days I waited for the slime mould to grow the more I began to die and go mouldy to the point where I had to throw away all the Agar dishes because they were very contaminated with mould.

Week commencing 04/01/2021

Plans for slime mould progression

-Working with coloured Slime mould?

I began to explore how I could finalise all my research and come up with a final outcome so I stared to reflect on the work I had done and also look for visual inspiration.

  • Inspiration one

I liked this digital manipulation of slime mould because of the variety of colours that were achieved. Screenshot 2021-01-07 at 10.44.44.png

  • Inspiration two

"Suzie, the Amazing Color Changing Slime Mold" - Slime mould that has been modified to be able to change colour!

Screenshot 2021-01-07 at 11.12.10.png Youtube

  • Inspiration three

How to dye slime mould?? "Hunter encouraged Physarum growth on silk and cotton in order to obtain a vibrant yellow fabric dye that was deposited by the movement of the plasmodium over the cloth, and also observed her other cultures bridging across agar to connect two ends of silver conductive thread together. She and Matheson dyed agar and oats with food colouring, and obtained stunning photographs of the Physarum's colour uptake and visible changes when presented with the altered media."

Colour.JPG

  • Inspiration four

Dying fabrics with Slime mould pigment

Screenshot 2021-01-07 at 11.55.19.png

Website link to Open Cell research - Dye with the Slime by Piero D'Angelo [1]

  • Inspiration five

Exploring the invisible - Colour (Prey) Preference in the Slime Mould Physarum polycephalum Weblink to the research page visualising the process [2]

Screenshot 2021-01-07 at 12.00.12.png

Week Commencing 11/01/2021

Next steps

  • Re-growing my slime mould when returning to Weimar, Introduce dying the Agar and the oats with food colouring to see if the slime mould changes colour as it eats this find source.
  • Buy an XXXXL Petri dish so that I can grow a large surface area of slime mould.
  • Exploring Zine ideas to create a slime mould zine


Zine Inspiration Research

Websites full of inspiration zines

All images are screenshots from the sources listed above

Week commencing 18/01/21

Dying experiments

Experimenting with dying the slime moulds environment. Will the slime mould adapt to it's environment and change colour also???

Process of dying the Agar

Clear agar with slime mould rapidly growing used incase the coloured slime mould didn't grow

Green dyed Agar and successfully growing slime mould


Red dyed agar and successfully growing slime mould

Blue dyed agar and successfully growing slime mould. Here you can also see that the slime mould is starting to change blue as a result of living within the blue environment.

Yellow dyed agar

Looking at the slime mould through the phone microscope

Looking at the slime mould with a DIY light under the petri-dish

Scanning experiments

Using a portable scanner I played around with the composition, colour and "glitchiness" of the results to create interesting manipulations.

Slime mould narrative for the Zine publication

Border of slime mould

Week commencing 25/01/21

Clear Agar with slime growing

Green Slime mould growing

Red slime mould growing

Blue slime mould growing

Yellow slime mould growing

Developed plan for the zine

Why did I chose to work with slime mould?

Because when I discovered it I was so interested by this micro-organism that was growing before my eyes! It was something so visual that I felt captivated and intrigued by it. Because of this the narrative of my zine will be about the discovery of slime mould.

Zine narrative - Discovery of slime mould

Zine will include

  • My initial images of visualising slime mould
  • My colour dying Agar experiments
  • Other peoples interpretations of what they think slime mould is (without knowing what it is)
  • Haiku poems
  • Questions
  • My scanned slime mould experiments
  • Analogue collaging