GMU:DIY Bio: doing things with biology/Paola Stephania Calderón: Difference between revisions

Latest revision as of 10:51, 3 April 2019

INTRODUCCTION

In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made two projects. The project idea 1: (teaching Physarum Polycephalum to live in salinity mediums) is the result of the knowledge that was acquired around the selected specimen Physarum Polycephalum in the class DIY: Bio: doing things with biology and the experimentation made in the class Experimental microbiology. The project idea 2: (insolation of gluconacetobacter with coral shape) is the result of the knowledge build during the class DIY: Bio: doing things with biology. During this class, I developed other experiments that build my knowledge around the work inside the laboratory, the cultivation of different kinds of specimens and techniques around the lab. work.

PROJECT IDEA 1

The idea is to compare how the specimen Physarum Polycephalum grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

62h Physarum Polycephalum growth in neutral environment video

52h Physarum Polycephalum growth in salinity mediums video (from 0% to 0.5% salinity)

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.

The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU: Experimental Microbiology, the experiment, and results can be seen in the following link: [1]

All experiments made in the class "Experimental Microbiology" can be seen at:

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> First approximation to the organism

The initial approximation to the specimen was made in the class: DIY BIO: doing things with biology. In this class three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of Physarum Polycephalum:

Links to further experiments with Physarum Polycephalum:

/Farming 1 Farming from a living sample

/Farming 2 Farming from a sleeping sample

/Farming 3 Farming from a combined sample

>> Second approximation to the organism

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

/20.11.2018 Growing Polycephalum/physarum from Caicedos investigation

PROJECT IDEA 2

The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

The diary of work made in the first approximation to isolate a specimen (with coral shape) can be seen in the following link.

/30.10.2018 Separation of one specimen - kombucha Link to the first approximation to this experiment.

FURTHER EXPERIMENTS

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

These experiments can be seen in the following links:

/16.10.2018 Introduction of the course

/06.11.2018a Growing Mycelium

/06.11.2018b Capturing electric potentials in organic matter

/18.12.2018 Observation of specimens under the microscope

/22.01.2019 Gelelectrophoresis

@@ Line 1: / Line 1: @@
-[[:File:Bio-02a4.png]]===='''*16.10.2018'''====
+==INTRODUCCTION==
-** General introduction of the course and the working norms inside the laboratory.
-** Lectures: ''The new vision 1928 fourth revised edition 1947 and abstract of an Artist'' by László Moholy-Nagy & ''Generation M. Matter, Markers, Microbiomes::Compost for Gaia.'' Dimitris Papadopoulos.
-** Preparation of the environment for the first bacteria crop in Petri box. ''two fingerprints and an A figure with the sample bacterias of Nizar mouth.
-[[File:Bio-01a.jpg|400px]]
-===='''*23.10.2018'''====
+In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made two projects. The '''project idea 1: ''(teaching Physarum Polycephalum to live in salinity mediums)'' ''' is the result of the knowledge that was acquired around the selected specimen ''Physarum Polycephalum'' in the class ''DIY: Bio: doing things with biology'' and the experimentation made in the class ''Experimental microbiology''. The '''project idea 2: ''(insolation of gluconacetobacter with coral shape)'' ''' is the result of the knowledge build during the class ''DIY: Bio: doing things with biology''. During this class, I developed other experiments that build my knowledge around the work inside the laboratory, the cultivation of different kinds of specimens and techniques around the lab. work.
-** Diskussion about the lectures from the first class.
-** Presentation about etichs.
-** Observation under microskope of existing specimens in the laboratory.
-[[File:Bio-01c.jpg|400px]]
-** Farming of new specimen in Petribox. ''Polycephalum/physarum''. The first one with living specimens, the second one with sleeping specimens. After the first night, they started to grow.
-[[File:Bio-02a.jpg|400px]]          [[File:Bio-02b.jpg|400px]]
-** Day 2, both specimens grow up in the darkness with a temperature of 20°C. The first specimens were closer to the food and have 5 leaflets, and they started to grow on top of it. The second specimens were farther from the food, and have just 3 leaflets, they grew in the direction of the food.
-[[File:Bio-02a01.jpg|400px]]          [[File:Bio-02b01.jpg|400px]]
-** Day 4 Just one of the specimens in the first Petri box seems to be growing out of the food. Both specimens that have grown a lot on the second day seemed to be dying inside the second Petri-box, probably there is not enough food for them now, or there's a specimen that contaminated the sample.
-[[File:Bio-02a2.png|400px]]          [[File:Bio-02b2.png|400px]]
-** Day 5 The specimens continue growing out of the food in the first Petri-box. In the second Petri-box what seems as dying was false, the specimens start to grow in the white color I'm waiting to see if they acquire the yellow color.
+==PROJECT IDEA 1==
-[[File:Bio-02a3.png|400px]]          [[File:Bio-02b3.png|400px]]
-** Day 6 The specimens in the first Petri-box haven´t changed their behavior, but definitely, there was something that contaminated the sample because is another specimen inside it. In the second Petri box is a concentration of the Polycephalum is in one side of the Petri-box, the tree growing specimen still have the white color.
+The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.
-[[File:Bio-02a4.png|400px]]          [[File:Bio-02b4.png|400px]]
-** Day 7 The second group of bacteria was made from one extreme with the living specimens, from the other one with sleeping specimens and the food was placed in the middle of the Petri-box. After one week both bacterias growth in direction of the food and then mixed up. A hypothesis about its big growing compared with the specimens that were located in my room, is that they haven't had the presence of light in one week. More specific results can be seen under the microscope.
+A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.
-[[File:Bio-02ca.png|400px]]
+[[File:62h normal medium.mp4|1500px]]
+h Physarum Polycephalum growth in neutral environment video
+[[File:52h salinity medium.mp4|1500px]]
+h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''
+Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
+<gallery>
+File:0% salinity.png
+File:0.062% salinity.png
+File:0.125% salinity.png
+File:0.25% salinity.png
+File:0.5% salinity.png
+File:1% salinity.png
+</gallery>
+''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU: Experimental Microbiology, the experiment, and results can be seen in the following link:   [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]
+''All experiments made in the class "Experimental Microbiology" can be seen at:''
+https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n
+RELATED EXPERIMENTS
+>> ''First approximation to the organism''
+The initial approximation to the specimen was made in the class: ''DIY BIO: doing things with biology''. In this class three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.
+Datasheet of ''Physarum Polycephalum:''
+[[File:DATA sheet Physarum Polycephalum.jpg|800px]]
+Links to further experiments with Physarum Polycephalum:
+[[/Farming 1]]  ''Farming from a living sample''
+[[/Farming 2]]  ''Farming from a sleeping sample''
+[[/Farming 3]]  ''Farming from a combined sample''
+>> ''Second approximation to the organism''
+This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.
+[[/20.11.2018]]  ''Growing Polycephalum/physarum from Caicedos investigation''
+==PROJECT IDEA 2==
+The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.
+The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.
+[[File:Gluconacetobacter guide.jpg|800px]]
+The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.
+[[/30.10.2018]]  ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.
+==FURTHER EXPERIMENTS==
+During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.
+''These experiments can be seen in the following links:''
+[[/16.10.2018]]  ''Introduction of the course''
+[[/06.11.2018a]]  ''Growing Mycelium''
+[[/06.11.2018b]]  ''Capturing electric potentials in organic matter''
+[[/18.12.2018]]    ''Observation of specimens under the microscope''
+[[/22.01.2019]]    ''Gelelectrophoresis''