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GMU:DIY Biolab “Driver’s License”/Jan Georg Glöckner/The digital lab-book: Difference between revisions
GMU:DIY Biolab “Driver’s License”/Jan Georg Glöckner/The digital lab-book (view source)
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Cloned 2 specimen of oyster mushrooms obtained from globus supermarket. The mushrooms are produced in Poland. | Cloned 2 specimen of oyster mushrooms obtained from globus supermarket. The mushrooms are produced in Poland. | ||
4 dishes were inoculated with specimen A. 3 dishes with specimen B. They are incubated at room temperature ( | 4 dishes were inoculated with specimen A. 3 dishes with specimen B. They are incubated at room temperature ( 23°C) | ||
autoclaved the horrible jar of penicilium. | autoclaved the horrible jar of penicilium. | ||
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'''26. January''' | '''26. January''' | ||
E³ shows aggressive leap off. | |||
Inoculated two long-storage-slants with the Brown Button Mushroom mycelium ( | Inoculated two long-storage-slants with the Brown Button Mushroom mycelium (E²). | ||
The old Pleurotus culture on cardboard gives mushrooms. | The old Pleurotus culture on cardboard gives mushrooms. | ||
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'''24. January''' | '''24. January''' | ||
Transferred rhyzomorph mycelium to | Transferred rhyzomorph mycelium to E³. | ||
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'''23. January''' | '''23. January''' | ||
E² shows rhyzomorph growth. | |||
Blanks are clean. | Blanks are clean. | ||
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'''18. January''' | '''18. January''' | ||
E² shows excellent leap of. | |||
Blanks are clean. | Blanks are clean. | ||
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Poured and sterilized plates. | Poured and sterilized plates. | ||
E² is growing rapidly. | |||
Two blanks are incubated to see if there is any contamination introduced through bad sterile-technique. | Two blanks are incubated to see if there is any contamination introduced through bad sterile-technique. | ||
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all dishes save E, are contaminated and the mycelium has died. | all dishes save E, are contaminated and the mycelium has died. | ||
E sports cottony mycelium and it seems like primordia forming, there is no contamination. | E sports cottony mycelium and it seems like primordia forming, there is no contamination. | ||
The mycelium from E is transferred to another dish labelled | The mycelium from E is transferred to another dish labelled E². | ||
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'''13. December''' | '''13. December''' | ||
Button Mushroom cloning. dishes showing mycelial leap of on the medium: A. B. C. D. E. Dishes showing no | Button Mushroom cloning. dishes showing mycelial leap of on the medium: A. B. C. D. E. Dishes showing no myceliuml growth whatsoever: G. | ||
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'''3. December''' | '''3. December''' | ||
Placed one of the G1 Masters in the incubator at | Placed one of the G1 Masters in the incubator at 25°C. | ||
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Pleurotus in malt-medium spawns cottony mycelium. The sample shows mild contamination. | Pleurotus in malt-medium spawns cottony mycelium. The sample shows mild contamination. | ||
the tupperware sample still is, if growing very slowly but shows no signs of contamination. | the tupperware sample still is, if growing very slowly but shows no signs of contamination. | ||
the temperature of the incubator is now 25. | the temperature of the incubator is now 25.5°C | ||