GMU:DIY Biolab “Driver’s License”/Jan Georg Glöckner/The digital lab-book: Difference between revisions

GMU:DIY Biolab “Driver’s License”/Jan Georg Glöckner/The digital lab-book (view source)

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Jan Glöckner

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-. December
+'''28. January'''
+Cloned 2 specimen of oyster mushrooms obtained from globus supermarket. The mushrooms are produced in Poland.
+dishes were inoculated with specimen A. 3 dishes with specimen B. They are incubated at room temperature ( 23 C)
+autoclaved the horrible jar of penicilium.
+'''26. January'''
+E3 shows aggresive leap off.
+Inoculated two long-storage-slants with the Brown Button Mushroom mycelium (E2).
+The old Pleurotus culture on cardboard gives mushrooms.
+Moved it from cold storage to the windowsill.
+'''24. January'''
+Transferred rhyzomorph mycelium to E3.
+'''23. January'''
+E2 shows rhyzomorph growth.
+Blanks are clean.
+'''18. January'''
+E2 shows excellent leap of.
+Blanks are clean.
+'''14. January'''
+Poured and sterilized plates.
+E2 is growing rapidly.
+Two blanks are incubated to see if there is any contamination introduced through bad sterile-technique.
+'''12. January'''
+Due to long illness all cultures are neglected:
+-all Grain-masters are over-incubated, None is usable for further development.
+Through poor air-circulation (no shaking) anaerobic conditions prevented the mycelium running. The mycelium itself looks healthy, but green molds are sharing the habitat.
+The mycelium ranges in colour from white over purple to red.
+The Button Mushroom cloning:
+all dishes save E, are contaminated and the mycelium has died.
+E sports cottony mycelium and it seems like primordia forming, there is no contamination.
+The mycelium from E is transferred to another dish labelled E2.
+'''13. December'''
 Button Mushroom cloning. dishes showing mycelial leap of on the medium: A. B. C. D. E. Dishes showing no mycelial growth whatsoever: G.