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''Materials'' | ''Materials'' | ||
[[File:materials.jpg|400px]] | |||
- 3 clean and disinfected petra dishes | - 3 clean and disinfected petra dishes | ||
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AGAR 2 (100ml Nutrient): 100ml distilled water - 2g glucose - 0,5g peptone - 0,5g yeast extract - 0,27g Na2HPO4 - 2g Agar. | AGAR 2 (100ml Nutrient): 100ml distilled water - 2g glucose - 0,5g peptone - 0,5g yeast extract - 0,27g Na2HPO4 - 2g Agar. | ||
ALL THE EXPERIMENT SHUND BE DEVELOP IN THE INFECTION FREE | ALL THE EXPERIMENT SHUND BE DEVELOP IN THE INFECTION FREE AREA! | ||
[[File:disinfected area.jpg|400px]] | |||
'''Step 1''' | '''Step 1''' | ||
Cut the schemes on the foil and preserve in alcohol. | Cut the schemes on the foil and preserve in alcohol. | ||
[[File:cortar folio 2.jpg|400px]] | |||
[[File:cortar folio 2.jpg|400px]] | |||
[[File:cortar folio 3.jpg|400px]] | |||
'''Step 2''' | '''Step 2''' | ||
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Prepare AGAR 1, mix and heat until boiling. | Prepare AGAR 1, mix and heat until boiling. | ||
Put the AGAR 1 into the 3 disinfected petra dishes, add the schemes on the top, cover and wait until it draws. | Put the AGAR 1 into the 3 disinfected petra dishes, add the schemes on the top, cover and wait until it draws. | ||
[[File:agar1enplato.jpg|400px]] | |||
[[File:agar1confolio.jpg|400px]] | |||
[[File:agar1confolio2.jpg|400px]] | |||
[[File:2017-01-19-14-16-21-3261.jpg|400px]] | |||
'''Step 3''' | '''Step 3''' | ||
with the help of the blades and the tweezers cut the edges of the schemes in order to empty the design, cover and preserve | with the help of the blades and the tweezers cut the edges of the schemes in order to empty the design, cover and preserve. | ||
[[File:cortandopatron1.jpg|400px]] | |||
'''Step 4''' | '''Step 4''' |
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