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''Dead Life Live Life'' is a sound installation work that sonifies the movement of DNA. This work was inspired by the poetic movement of DNA in electrophoresis experiments. ''Dead Life Live Life'' explores the materiality of DNA itself rather than genetic analysis, which is the general purpose of DNA. “Dead life got the same material as live life”, biologist Lynn Margulis said in an interview. This could be paraphrased as “Dead life got the same DNA as live life”. ''Dead Life Live Life'' poetically represents the connection between life and death by sonifying the DNA movement of an old persimmon tree planted in my grandmother's house. | {{#ev:youtube|bGzmhJ9kQi4}} | ||
=== '''Dead Life Live Life (working title)''' === | |||
''Dead Life Live Life'' is a sound installation work that sonifies the movement of DNA. This work was inspired by the poetic movement of DNA in electrophoresis experiments. ''Dead Life Live Life'' explores the materiality of DNA itself rather than genetic analysis, which is the general purpose of DNA. “Dead life got the same material as live life”, biologist Lynn Margulis said in an interview. This could be paraphrased as “Dead life got the same DNA as live life”. ''Dead Life Live Life'' poetically represents the connection between life and death by sonifying the DNA movement of an old persimmon tree planted in my grandmother's house. | |||
[[File:Diagram Dead LifeLiveLife.jpg|center|thumb|800x800px]] | |||
[[File:Dlll 1.001.jpg|center|frameless|800x800px]] | [[File:Dlll 1.001.jpg|center|frameless|800x800px]] | ||
[[File:Dlll 2..jpg|center|frameless|800x800px]] | [[File:Dlll 2..jpg|center|frameless|800x800px]] | ||
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==== Technical solution ==== | ====Technical solution==== | ||
===== 1. Biological part ===== | =====1. Biological part===== | ||
Instruction of DNA analysis<gallery> | Instruction of DNA analysis<gallery> | ||
File:Instruction1 DNAworkshop.jpg | File:Instruction1 DNAworkshop.jpg | ||
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=====2. Sound part===== | |||
===== 2. Sound part ===== | |||
1) Sonification of DNA movementMax/Msp patch | 1) Sonification of DNA movementMax/Msp patch | ||
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2) Sound Design | 2) Sound Design | ||
==== Work in Biolab ==== | ====Work in Biolab==== | ||
===== '''2024.11.16. Sat''' ===== | ====='''2024.11.16. Sat'''===== | ||
<u>Introduction</u> | <u>Introduction</u> | ||
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<gallery>File:After 50hours gelElectrophoresis.jpg</gallery> | <gallery>File:After 50hours gelElectrophoresis.jpg</gallery> | ||
===== ''' 2024.11.18. Mon''' ===== | =====''' 2024.11.18. Mon'''===== | ||
<u>Introduction</u> | <u>Introduction</u> | ||
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<gallery> File:DNALadder UV lighting.jpg </gallery> | <gallery> File:DNALadder UV lighting.jpg </gallery> | ||
===== '''2024.11.19. Tue''' ===== | ====='''2024.11.19. Tue'''===== | ||
<u>Introduction</u> | <u>Introduction</u> | ||
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</gallery> | </gallery> | ||
===== '''2024.11.20. Wed''' ===== | ====='''2024.11.20. Wed'''===== | ||
<u>Introduction</u> | <u>Introduction</u> | ||
DNA Extraction Exercise_ Kaki | DNA Extraction Exercise_ Kaki, Leaf | ||
- I failed to extract Kaki's DNA in my experiment yesterday. This time i followed | - I failed to extract Kaki's DNA in my experiment yesterday. This time i followed the guide from the result of research by Alessandro to extract Kaki's DNA. | ||
- Alessandro's research results show that DNA is mainly extracted from the leaves of the persimmon. Since I can't get the leaves of the persimmon right now, I tried the experiment with the weeds on the roadside. | - Alessandro's research results show that DNA is mainly extracted from the leaves of the persimmon. Since I can't get the leaves of the persimmon right now, I tried the experiment with the weeds on the roadside. | ||
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-The DNA extraction experiment was performed following the guide of chat gpt for the persimmon and the existing instructions for the leaf. | -The DNA extraction experiment was performed following the guide of chat gpt for the persimmon and the existing instructions for the leaf. | ||
<small>To extract DNA from kaki (persimmon) fruit, you'll need about '''3-4 fruits''' to get a sufficient amount of DNA for analysis. Here's a step-by-step procedure:</small> | <small>To extract DNA from kaki (persimmon) fruit, you'll need about '''3-4 fruits''' to get a sufficient amount of DNA for analysis. Here's a step-by-step procedure:</small> | ||
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Both the leaves and the persimmon failed to extract DNA. I was a little frustrated. | Both the leaves and the persimmon failed to extract DNA. I was a little frustrated. | ||
It seems like I need to extract DNA from the leaves persimmon tree. | |||
But where do I get the leaves?<gallery> | But where do I get the leaves?<gallery> | ||
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</gallery> | </gallery> | ||
===== '''2024.11.21. Thu''' ===== | ====='''2024.11.21. Thu'''===== | ||
<u>Introduction</u> | <u>Introduction</u> | ||
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I followed the instruction in Julian's workshop for setting up the centrifugation of DNA sample and PCR. | I followed the instruction in Julian's workshop for setting up the centrifugation of DNA sample and PCR. | ||
# Centrifuge setting: 8kg, 60 seconds | #Centrifuge setting: 8kg, 60 seconds | ||
# PCR: see instructional photo above. | #PCR: see instructional photo above. | ||
A total of five DNA samples were subjected to DNA amplification by PCR | A total of five DNA samples were subjected to DNA amplification by PCR | ||
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*#B2: DNA of Blueberries (from 21.Nov) | *#B2: DNA of Blueberries (from 21.Nov) | ||
*#BA: DNA of Banana (from 21.Nov) | *#BA: DNA of Banana (from 21.Nov) | ||
*#K: Kaki pulp with soap, salt, hot water (from 20.Nov) | *# K: Kaki pulp with soap, salt, hot water (from 20.Nov) | ||
<small>*sample B11, B12 are dirtier than B2</small> | <small>*sample B11, B12 are dirtier than B2</small> | ||
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<nowiki></gallery> | <nowiki></gallery> | ||
===== '''2024.11.26. Tue''' ===== | ====='''2024.11.26. Tue'''===== | ||
<u>Introduction</u> | <u>Introduction</u> | ||
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