GMU:Cybernetic Subjects - Ursula Damm (Georg Trogemann)/Dahye Seo: Difference between revisions

GMU:Cybernetic Subjects - Ursula Damm (Georg Trogemann)/Dahye Seo (view source)

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Dahyeseo

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 File:DNA Extraction Banana-blueberry2.jpg
 </gallery><u>Result</u>
 DNA extraction was successful for both bananas and blueberries. DNA extraction is particularly easy for bananas. Blueberries, like strawberries and raspberries, do not have a high DNA extraction rate compared to other berry types.<gallery>
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 File:DNA from blueberry in tube.jpg
 File:DNA from Banana in tube.jpg
+</gallery>
+====='''2024.11.23. Sat'''=====
+<u>Introduction</u>
+Exercise for DNA centrifugation and PCR Experiment
+<u>Procedure</u>
+I followed the instruction in Julian's workshop for setting up the centrifugation of DNA sample and PCR.
+# Centrifuge setting: 8kg, 60 seconds
+# PCR: see instructional photo above.
+A total of five DNA samples were subjected to DNA amplification by PCR
+*Label information
+*#B11: DNA of Blueberries (from 19.Nov)
+*#B12: DNA of Blueberries (from 19.Nov)
+*#B2:  DNA of Blueberries (from 21.Nov)
+*#BA: DNA of Banana (from 21.Nov)
+*#K: Kaki pulp with soap, salt, hot water (from 20.Nov)
+<small>*sample B11, B12  are dirtier than B2</small>
+<small>*So far no success to extract Kaki DNA. I used this time kaki pulp instead of DNA. It seems like there are no DNA in pulp, but at least I wanted experiment with pulp until Gel-electrophoresis just for double checking.  </small>
+<gallery>
+File:Centrifugation1.jpg
+File:Material for pcr.jpg
+File:Pcr1.jpg
+File:Pcr2.jpg
+File:Pcr setting bento machine.jpg
+<nowiki></gallery>
+===== '''2024.11.26. Tue''' =====
+<u>Introduction</u>
+Extracting DNA from leaves using DNeasy Kit with supervising by  Alessandro Volpato_ Since there are no leaves from persimmon trees, I experimented with leaves from other trees in Ilm park.
+<u>Procedure</u>
+The protocol below was followed.
+[[File:DNeasy protocol.pdf|left|thumb]]
+<nowiki>https://youtu.be/c1WOBy5vSg8?si=ksFltpYkG1ytvugT</nowiki>
+<gallery>
+File:Dneasy kit1.jpg
+File:Dneasy kit.jpg
+File:Dneasy kit2.jpg
+File:Dneasy kit3.jpg
+File:Dneasy kit4.jpg
+File:Dneasy kit5.jpg
+File:Dneasy kit6.jpg
 </gallery>
+<u>Result</u>
+I followed the protocol to extract DNA from the leaves, but I could not visually check if the DNA was extracted correctly. After the gel electrophoresis, i I will know if the DNA was extracted. �I will start electrophoresis as soon as the Stein green arrives.