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====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes==== | ====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes==== | ||
<gallery> | ::<gallery> | ||
File:Mannitol.png | File:Mannitol.png | ||
File:Plates.png | File:Plates.png | ||
</gallery> | </gallery> | ||
< | |||
<tt> | |||
MSA RECIPE: | MSA RECIPE: | ||
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10 mM CaCl2 | 10 mM CaCl2 | ||
100 mL Deionised water | 100 mL Deionised water | ||
Medium alternative: Inorganic Salt Starch Agar (ISSA) | Medium alternative: Inorganic Salt Starch Agar (ISSA) | ||
</ | </tt> | ||
====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)==== | ====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)==== |
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