The human gets ill, goes downhill, forgets the dollar bill, takes a pill, regains will, re-feels the thrill.
It’s simply the recuperation cycle.
But, do you ever think about the fundamentals of having the chance of recuperation from infections?
It’s thanks to the pill - the antibiotic.
Now, zoom in deeper…
Do you actually know where this savior comes from?
Think no further than the soil.
In a 250 mL conical flask mix:
Medium alternative: Inorganic Salt Starch Agar (ISSA)
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
Important: Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination.
Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
Observations: The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.
Observations: There has been overgrowing a hybrid of organisms, but assumingly the pinky/white powdery dots are streptomyces colonies.Such colonies are mostly to be found on the 1:100 and 1:1000 dilution plates. In this case, creating subcultures is the best solution, in order to get a unified-type of biotope in the petri dish!
Only one sample has been contaminated, as it was the first one to get done during the isolation process.
Streptomyces species are the most abundant source of antibiotics. More specifically, they produce the two-thirds of all existent antibiotics. Except that, this genus produces a rife scope of bioactive compounds. It usually inhabits the soil and plays a significant role in decomposing.
So, next time when you get ill, decide to take another pill, in order to re-feel the thrill...
...take a minute to think and praise the genus of good welfare.