Revision as of 22:20, 12 July 2016

Spot the antibiotics producer: STREPTOMYCES

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Antibiotics.

The human gets ill, goes downhill, forgets the dollar bill, takes a pill, regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than the soil.

It’s Streptomyces.

Isolation of Streptomyces

1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment

2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min

3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it

4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes

MSA RECIPE:

In a 250 mL conical flask mix:
2g Agar
2g Mannitol
2g Soya flour (Holland and Barrett)
10 mM CaCl2
100 mL Deionised water

Medium alternative: Inorganic Salt Starch Agar (ISSA)

5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)

6th step: Incubate at 30° for 5-7 days

Inspiration: http://annadumitriu.tumblr.com/ - Anna Dumitriu https://exploringtheinvisible.com/ - Dr. Simon Park

@@ Line 40: / Line 40: @@
 :'''4th step''': Prepare the Mannitol Soy Agar (MSA) petri dishes
+<pre>
+MSA RECIPE:
-''MSA Recipe:''
+In a 250 mL conical flask mix:
+g Agar
-In a '''250 mL conical flask''' mix:
+g Mannitol
-*2g Agar
+g Soya flour (Holland and Barrett)
-*2g Mannitol
+mM CaCl2
-*2g Soya flour (Holland and Barrett)
+mL Deionised water
-*10 mM CaCl2
-*100 mL Deionised water
 Medium alternative: Inorganic Salt Starch Agar (ISSA)
+</pre>
 :'''5th step''': Plate an aliquot of ''0.1ml of each dilution'' on the medium (MSA)