GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata: Difference between revisions

Revision as of 22:02, 12 July 2016

STREPTOMYCES

SPOT THE ANTIBIOTICS PRODUCER

Micro1.png
Micro3.png
Micro4.png

Antibiotics.

The human gets ill, goes downhill, forgets the dollar bill, takes a pill, regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than the soil.

It’s Streptomyces.

Inspiration: http://annadumitriu.tumblr.com/ - Anna Dumitriu https://exploringtheinvisible.com/ - Dr. Simon Park

@@ Line 1: / Line 1: @@
-== A visual representation of bacterial communication systems ==
+==STREPTOMYCES==
+===SPOT THE '''ANTIBIOTICS PRODUCER'''===
+<gallery>
+File:Micro1.png
+File:Micro2.png
+File:Micro3.png
+File:Micro4.png
+</gallery>
-''How do living organisms communicate and connect with the external? How is that structured and how much of it is comparable to the communication networks of the non-living? Is it valid to state that the communication networks of modern infrastructures are a projection of the communication networks built among the living?''
+''Antibiotics''.
-===STREPTOMYCES===
+The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.
-====1st step: Streptomyces isolation from pure soil====
+It’s simply the recuperation cycle.
-I. Air dry the soil samples for '''3-4 h at 45°''' to select for Actinomyces spores, considering that many other bacteria die during this pre-treatment
+But, do you ever think about the fundamentals of having the chance of recuperation from infections?
+Sure.
-[[Image:Soil.png|200px]]
+It’s thanks to the pill - the antibiotic.
-II. Plate the samples on to '''Mannitol Soy Flour Agar (MSA)'''
+Good job!
-''Necessities for the recipe of Mannitol Soy Flour Agar''
+Now, zoom in deeper…
-[[Image:Mannitol.png|200px]]
+Do you actually know where this savior comes from?
-In a 250 mL conical flask mix:
+Think no further than ''the soil''.
-* 2g Agar
-* 2g Mannitol
-* 2g Soya flour (Holland and Barrett)
-* 10 mM CaCl2
-* 100 mL Deionised water
+It’s ''Streptomyces''.
-III. Incubate at '''30°C for 7 days'''
-[[Image:Incubation.png|400px]]
-Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
-====2nd step: Adding nutritive elements====
-A variety of nutritive elements, such as ''sugar, oats, yeast'' etc. is added in order to identify the selective abilities and possible communication pathways of Streptomyces when it comes to nutrition.
-===PAENIBACILLUS VORTEX===
-[[Image:Vortexx.png|middle|250px]]
-Considering the pattern-forming abilities of ''Paenibacillus Vortex'', I am interested in depicting the possible networks this bacterium can build through quorum sensing, when it comes to the presence of nutrition. Known as one of the most intelligent bacterium species due to its advanced quorum sensing, PV might as well be the proper organism to give clear and differential communication structures.
-====1st step: Medium prep====
-In a 250mL conical flask mix:
-*3g agar
-*4g peptone
-*200 mL distilled water
-[[Image:PVagar.png|200px|thumb|left|Nutritive Agar]]
-====2nd step: Isolation====
-====3rd step: Incubation====
-''The bacterial strain of Paenibacillus Vortex was obtained from the Microbiology Research Institute of the Friedrich-Schiller University in Jena.''
 '''Inspiration''':
-http://orielisar.com/ - Ori Elisar
-http://www.nuritbarshai.com/objectivity/ - Nurit Bar-Shai
 http://annadumitriu.tumblr.com/ - Anna Dumitriu
 https://exploringtheinvisible.com/ - Dr. Simon Park