GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata: Difference between revisions
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I. Air dry the soil samples for '''3-4 h at 45°''' to select for Actinomyces spores, considering that many other bacteria die during this pre-treatment | I. Air dry the soil samples for '''3-4 h at 45°''' to select for Actinomyces spores, considering that many other bacteria die during this pre-treatment | ||
II. Plate the samples either on to ''' | II. Plate the samples either on to '''Mannitol Soy Flour Agar (MSA)''' | ||
''Necessities for the recipe of Mannitol Soy Flour Agar'' | ''Necessities for the recipe of Mannitol Soy Flour Agar'' | ||
| Line 19: | Line 19: | ||
* 10 mM CaCl2 | * 10 mM CaCl2 | ||
* 100 mL Deionised water | * 100 mL Deionised water | ||
III. Incubate at '''30°C for 7 days''' | III. Incubate at '''30°C for 7 days''' | ||
| Line 24: | Line 25: | ||
Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria | Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria | ||
====2nd step: Adding | ====2nd step: Adding nutritive elements==== | ||
A variety of nutritive elements, such as ''sugar, oats, yeast'' etc. is added in order to identify the selective abilities and possible communication pathways of Streptomyces when it comes to nutrition. | |||
===PAENIBACILLUS VORTEX=== | ===PAENIBACILLUS VORTEX=== | ||
[[Image:Vortexx.png|250px]] | [[Image:Vortexx.png|250px]] | ||
# Considering the pattern-forming abilities of ''Paenibacillus Vortex'', I am interested in depicting the possible networks this bacterium can build through quorum sensing, when it comes to the presence of nutrition. Known as one of the most intelligent bacterium species due to its advanced quorum sensing, PV might as well be the proper organism to give clear and differential communication structures. | |||
===1st step: Medium prep=== | |||
In a 250mL conical flask mix: | |||
*3g agar | |||
*4g peptone | |||
*200 mL distilled water | |||
[[Image:PVagar.png|200px|thumb|right|Nutritive Agar]] | |||
====1st step: Isolation and incubation==== | ====1st step: Isolation and incubation==== | ||
====2nd step: Adding Pepton==== | ====2nd step: Adding Pepton==== | ||
''The bacterial strain of Paenibacillus Vortex was obtained from the Microbiology Research Institute of the Friedrich-Schiller University in Jena.'' | |||
'''Inspiration''': | '''Inspiration''': | ||
Revision as of 11:04, 7 July 2016
A visual representation of bacterial communication systems
How do living organisms communicate and connect with the external? How is that structured and how much of it is comparable to the communication networks of the non-living? Is it valid to state that the communication networks of modern infrastructures are a projection of the communication networks built among the living?
STREPTOMYCES
1st step: Streptomyces isolation from pure soil
I. Air dry the soil samples for 3-4 h at 45° to select for Actinomyces spores, considering that many other bacteria die during this pre-treatment
II. Plate the samples either on to Mannitol Soy Flour Agar (MSA)
Necessities for the recipe of Mannitol Soy Flour Agar
In a 250 mL conical flask mix:
- 2g Agar
- 2g Mannitol
- 2g Soya flour (Holland and Barrett)
- 10 mM CaCl2
- 100 mL Deionised water
III. Incubate at 30°C for 7 days
Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
2nd step: Adding nutritive elements
A variety of nutritive elements, such as sugar, oats, yeast etc. is added in order to identify the selective abilities and possible communication pathways of Streptomyces when it comes to nutrition.
PAENIBACILLUS VORTEX
- Considering the pattern-forming abilities of Paenibacillus Vortex, I am interested in depicting the possible networks this bacterium can build through quorum sensing, when it comes to the presence of nutrition. Known as one of the most intelligent bacterium species due to its advanced quorum sensing, PV might as well be the proper organism to give clear and differential communication structures.
1st step: Medium prep
In a 250mL conical flask mix:
- 3g agar
- 4g peptone
- 200 mL distilled water
1st step: Isolation and incubation
2nd step: Adding Pepton
The bacterial strain of Paenibacillus Vortex was obtained from the Microbiology Research Institute of the Friedrich-Schiller University in Jena.
Inspiration:
http://orielisar.com/ - Ori Elisar
http://www.nuritbarshai.com/objectivity/ - Nurit Bar-Shai
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park