GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata: Difference between revisions
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II. Plate the samples either on to '''Actinomycete Isolation Agar (AIA) or Mannitol Soy Flour Agar (MSA)''' | II. Plate the samples either on to '''Actinomycete Isolation Agar (AIA) or Mannitol Soy Flour Agar (MSA)''' | ||
''Necessities for the recipe of Mannitol Soy Flour Agar'' | |||
In a 250 mL conical flask mix: | |||
* 2g Agar | |||
* 2g Mannitol | |||
* 2g Soya flour (Holland and Barrett) | |||
* 10 mM CaCl2 | |||
* 100 mL Deionised water | |||
III. Incubate at '''30°C for 7 days''' | III. Incubate at '''30°C for 7 days''' | ||
Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria | Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria | ||
# Considering the pattern-forming abilities of ''Paenibacillus Vortex'', I am interested in depicting the communication pathways between nutrients and bacteria. Basically, how the bacteria reach for its nutrients. I am planning on isolating the ''Paenibacillus Vortex'' on agar and adding ''Pepton'' as a nutrient. | # Considering the pattern-forming abilities of ''Paenibacillus Vortex'', I am interested in depicting the communication pathways between nutrients and bacteria. Basically, how the bacteria reach for its nutrients. I am planning on isolating the ''Paenibacillus Vortex'' on agar and adding ''Pepton'' as a nutrient. | ||
Revision as of 21:00, 4 July 2016
After a while of research, I decided to focus on isolating distinct types of bacteria with the aim showing their inter-communication regarding food, as well as their dyeing abilities. Indeed, this idea is not electronics-related, but the good news is, that the name of this course has also the “aesthetics and other interesting stuff” part on it.
A visual representation of bacterial communication systems
Streptomyces isolation from pure soil
I. Air dry the soil samples for 3-4 h at 45° to select for Actinomyces spores, considering that many other bacteria die during this pre-treatment
II. Plate the samples either on to Actinomycete Isolation Agar (AIA) or Mannitol Soy Flour Agar (MSA)
Necessities for the recipe of Mannitol Soy Flour Agar
In a 250 mL conical flask mix:
- 2g Agar
- 2g Mannitol
- 2g Soya flour (Holland and Barrett)
- 10 mM CaCl2
- 100 mL Deionised water
III. Incubate at 30°C for 7 days
Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
- Considering the pattern-forming abilities of Paenibacillus Vortex, I am interested in depicting the communication pathways between nutrients and bacteria. Basically, how the bacteria reach for its nutrients. I am planning on isolating the Paenibacillus Vortex on agar and adding Pepton as a nutrient.