GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata: Difference between revisions

Revision as of 20:45, 4 July 2016

After a while of research, I decided to focus on isolating distinct types of bacteria with the aim showing their inter-communication regarding food, as well as their dyeing abilities. Indeed, this idea is not electronics-related, but the good news is, that the name of this course has also the “aesthetics and other interesting stuff” part on it.

A visual representation of bacterial communication systems

Streptomyces isolation from pure soil

I. Air dry the soil samples for 3-4 h at 45° to select for Actinomyces spores, considering that many other bacteria die during this pre-treatment

II. Plate the samples either on to Actinomycete Isolation Agar (AIA) or Mannitol Soy Agar (MSA)

III. Incubate at 30°C for 7 days

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria

Considering the pattern-forming abilities of Paenibacillus Vortex, I am interested in depicting the communication pathways between nutrients and bacteria. Basically, how the bacteria reach for its nutrients. I am planning on isolating the Paenibacillus Vortex on agar and adding Pepton as a nutrient.

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 After a while of research, I decided to focus on isolating distinct types of bacteria with the aim showing their inter-communication regarding food, as well as their dyeing abilities. Indeed, this idea is not electronics-related, but the good news is, that the name of this course has also the “aesthetics and other interesting stuff” part on it.
-=== Main Ideas ===
+== A visual representation of bacterial communication systems ==
-# Considering the pattern-forming abilities of ''Paenibacillus Vortex'', I am interested in depicting the communication pathways between nutrients and bacteria. Basically, how the bacteria reach for its nutrients. I am planning on isolating the ''Paenibacillus Vortex'' on agar and adding ''Pepton'' as a nutrient.
+# Streptomyces isolation from pure soil
-::What’s missing: I am on the process of getting the correct information on where to get this bacterium. After that, work will be ready to get started with.
+I. Air dry the soil samples for '''3-4 h at 45°''' to select for Actinomyces spores, considering that many other bacteria die during this pre-treatment
+II. Plate the samples either on to '''Actinomycete Isolation Agar (AIA) or Mannitol Soy Agar (MSA)'''
-# Ecologically-thinking, I am also interested in working with ''Streptomyces'' – the bacterium from which approx. 70% of the antibiotics is created. Since it grows in beautiful colors, I plan on trying to isolate it on ''ISSA'' (Inorganic Salt Starch Agar), on top of a texture (could be silk). Thus I can try to see whether it is capable of being used as a substitution of texture dyes or not.
+III. Incubate at '''30°C for 7 days'''
-:: What’s missing: Still waiting to get an answer, whether there is ISSA on BioLab. This could be also found in agricultural pharmacies, but apparently there is no such pharmacy here in Weimar.
+Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
-''later on I will be posting my sketches, photos and lists for the foreseen works!''
+# Considering the pattern-forming abilities of ''Paenibacillus Vortex'', I am interested in depicting the communication pathways between nutrients and bacteria. Basically, how the bacteria reach for its nutrients. I am planning on isolating the ''Paenibacillus Vortex'' on agar and adding ''Pepton'' as a nutrient.