GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata: Difference between revisions
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====6th step: Incubate at 30° for 5-7 days==== | ====6th step: Incubate at 30° for 5-7 days==== | ||
'''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination. | '''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination. | ||
[[File:Incubation.png|400px]] | [[File:Incubation.png|400px]] | ||
Revision as of 07:38, 13 July 2016
Spot the antibiotics producer: STREPTOMYCES
Antibiotics.
The human gets ill, goes downhill, forgets the dollar bill, takes a pill, regains will, re-feels the thrill.
It’s simply the recuperation cycle.
But, do you ever think about the fundamentals of having the chance of recuperation from infections?
Sure.
It’s thanks to the pill - the antibiotic.
Good job!
Now, zoom in deeper…
Do you actually know where this savior comes from?
Think no further than the soil.
Streptomyces.
ISOLATION OF STREPTOMYCES
1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment
2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min
3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it
4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes
MSA RECIPE:
In a 250 mL conical flask mix:
- 2g Agar
- 2g Mannitol
- 2g Soya flour (Holland and Barrett)
- 10 mM CaCl2
- 100 mL Deionised water
Medium alternative: Inorganic Salt Starch Agar (ISSA)
5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
6th step: Incubate at 30° for 5-7 days
Important:Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination.
Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
3-DAY OUTCOMES
Observations: The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.
6-DAY OUTCOMES
Inspiration:
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park