GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata: Difference between revisions
From Medien Wiki
mNo edit summary |
No edit summary |
||
Line 39: | Line 39: | ||
:'''3rd step''': Prepare 3 dilutions ''(1:10, 1:100, 1:1000)'' out of it | :'''3rd step''': Prepare 3 dilutions ''(1:10, 1:100, 1:1000)'' out of it | ||
:4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes | :'''4th step''': Prepare the Mannitol Soy Agar (MSA) petri dishes | ||
MSA Recipe: | ''MSA Recipe:'' | ||
In a '''250 mL conical flask''' mix: | In a '''250 mL conical flask''' mix: | ||
Line 50: | Line 50: | ||
*100 mL Deionised water | *100 mL Deionised water | ||
Medium alternative: Inorganic Salt Starch Agar (ISSA) | |||
:'''5th step''': Plate an aliquot of ''0.1ml of each dilution'' on the medium (MSA) | |||
:'''6th step''': Incubate at ''30° for 5-7 days'' | |||
---- | |||
'''Inspiration''': | '''Inspiration''': | ||
http://annadumitriu.tumblr.com/ - Anna Dumitriu | http://annadumitriu.tumblr.com/ - Anna Dumitriu | ||
https://exploringtheinvisible.com/ - Dr. Simon Park | https://exploringtheinvisible.com/ - Dr. Simon Park |
Revision as of 22:18, 12 July 2016
Spot the antibiotics producer: STREPTOMYCES
- Micro1.png
- Micro3.png
- Micro4.png
Antibiotics.
The human gets ill, goes downhill, forgets the dollar bill, takes a pill, regains will, re-feels the thrill.
It’s simply the recuperation cycle.
But, do you ever think about the fundamentals of having the chance of recuperation from infections?
Sure.
It’s thanks to the pill - the antibiotic.
Good job!
Now, zoom in deeper…
Do you actually know where this savior comes from?
Think no further than the soil.
It’s Streptomyces.
Isolation of Streptomyces
- 1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment
- 2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min
- 3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it
- 4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes
MSA Recipe:
In a 250 mL conical flask mix:
- 2g Agar
- 2g Mannitol
- 2g Soya flour (Holland and Barrett)
- 10 mM CaCl2
- 100 mL Deionised water
Medium alternative: Inorganic Salt Starch Agar (ISSA)
- 5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)
- 6th step: Incubate at 30° for 5-7 days
Inspiration: http://annadumitriu.tumblr.com/ - Anna Dumitriu https://exploringtheinvisible.com/ - Dr. Simon Park