Revision as of 22:14, 12 July 2016

Spot the antibiotics producer: STREPTOMYCES

Micro1.png
Micro3.png
Micro4.png

Antibiotics.

The human gets ill, goes downhill, forgets the dollar bill, takes a pill, regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than the soil.

It’s Streptomyces.

Isolation of Streptomyces

1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment

2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min

3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it

4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes

MSA Recipe:

In a 250 mL conical flask mix:

2g Agar
2g Mannitol
2g Soya flour (Holland and Barrett)
10 mM CaCl2
100 mL Deionised water

Inspiration: http://annadumitriu.tumblr.com/ - Anna Dumitriu https://exploringtheinvisible.com/ - Dr. Simon Park

@@ Line 32: / Line 32: @@
 ----
+===Isolation of Streptomyces===
+:'''1st step''': Air dry the soil samples for ''3-4 h at 45°'' for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment
+:'''2nd step''':Dilute ''1-3g of soil in 10 ml saline solution'' (NaCl 0,9%) and let the solution stand for ''15 min''
+:'''3rd step''': Prepare 3 dilutions ''(1:10, 1:100, 1:1000)'' out of it
+:4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes
+MSA Recipe:
+In a '''250 mL conical flask''' mix:
+*2g Agar
+*2g Mannitol
+*2g Soya flour (Holland and Barrett)
+*10 mM CaCl2
+*100 mL Deionised water
 '''Inspiration''':
 http://annadumitriu.tumblr.com/ - Anna Dumitriu
 https://exploringtheinvisible.com/ - Dr. Simon Park