GMU:DIY Bio: doing things with biology/Paola Stephania Calderón/30.10.2018: Difference between revisions
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* Distilled or tap water - 500ml | * Distilled or tap water - 500ml | ||
* Agar - 7.5g | * Agar - 7.5g | ||
2) Bacteria insolation, a mixture of original bacteria ''combuscha'' diluted in distilled water in different proportions. | 2) Bacteria insolation, a mixture of original bacteria ''combuscha'' diluted in distilled water in different proportions. | ||
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[[File:Bio-03c.png|400px]] | [[File:Bio-03c.png|400px]] | ||
* Day 8: In order to extract one specimen, 5 new Petri box were made like following; | |||
1) Medium preparation for ''Gluconacetobacter'' (100ml) | |||
* Glucose - 2g | |||
* Peptone - 0.5g | |||
* Yeast extract - 0.5g | |||
* Na2HPO4 - 0.27g | |||
* Citric acid - 0.15g | |||
* Distilled or tap water - 100ml | |||
* Agar - 1.5g | |||
2) Bacteria insolation, from each sample that was made in the first | |||
*1) |
Revision as of 13:51, 7 November 2018
- Discussion about Symbiosis.
- Presentation about projects that used microorganism. Examples with food from Christina Agapakis Bread made with death bacterias and another project with cheese and Transmediale Festival in Berlin 1000 Handshakes
- Laboratory project, insolation of one bactería from a group of bacterias.
- Day 1: Preparation of sample
1) Medium preparation for Gluconacetobacter (500ml)
- Glucose - 10g
- Peptone - 2.5g
- Yeast extract - 2.5g
- Na2HPO4 - 1.35g
- Citric acid - 0.75g
- Distilled or tap water - 500ml
- Agar - 7.5g
2) Bacteria insolation, a mixture of original bacteria combuscha diluted in distilled water in different proportions.
- 1) 5 parts of original bacteria into 50 parts of distilled water 1/10
- 2) 2 parts of sample #1 into 50 parts of distilled water.
- 3) 2 parts of sample #2 into 50 parts of distilled water.
- 4) 2 parts of sample #3 into 50 parts of distilled water.
- 5) 2 parts of sample #4 into 50 parts of distilled water.
- 6) Distilled water. control sample
- Day 4: after four days the bacterias seems to be growing, all of them in white color.
- Day 7: after one week the microorganism (bacteria) that is wanted to be insolated, start to grow, is the white circle spots that can be clearly seen in Petri number 3, 4 and 5.
- Day 8: In order to extract one specimen, 5 new Petri box were made like following;
1) Medium preparation for Gluconacetobacter (100ml)
- Glucose - 2g
- Peptone - 0.5g
- Yeast extract - 0.5g
- Na2HPO4 - 0.27g
- Citric acid - 0.15g
- Distilled or tap water - 100ml
- Agar - 1.5g
2) Bacteria insolation, from each sample that was made in the first
- 1)