GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata: Difference between revisions
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===ISOLATION OF STREPTOMYCES=== | ===ISOLATION OF STREPTOMYCES=== | ||
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment==== | =====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment===== | ||
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File:Soil1.png | File:Soil1.png | ||
File:Soil.png | File:Soil.png | ||
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====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min==== | ====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min==== | ||
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File:NACL.png | File:NACL.png | ||
File:Solution .png | File:Solution .png | ||
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====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it==== | ====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it==== | ||
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File:Diluting.png | File:Diluting.png | ||
File:Dilutions&Solution.png | File:Dilutions&Solution.png | ||
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====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes==== | ====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes==== | ||
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File:Mannitol.png | File:Mannitol.png | ||
File:Plates.png | File:Plates.png | ||