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=== Day 0 === | === Day 0 === | ||
I build and set up a box, seperated in 4 sections. Each section containing one 1 Watt LED to support the inhabitants with light. The boxes have fans, so the air inside will be interchanged from time to time. To control the amount and - for the first experiment - the duration of the light and the fans i wrote a little script that is running on an arduino. This script also counts the time (and in case of a powerdrain stores this information on the eeprom). | I build and set up a box, seperated in 4 sections. Each section containing one 1 Watt LED to support the inhabitants with light. The boxes have fans, so the air inside will be interchanged from time to time. To control the amount and - for the first experiment - the duration of the light and the fans i wrote a little [[:File:artiPlants_01.zip|script]] that is running on an arduino. This [[:File:artiPlants_01.zip|script]] also counts the time (and in case of a powerdrain stores this information on the eeprom). The LED are suspended from the top of the box using some bend metal strips, which simply clamp the star platine of the leds in place. Therefor i was able to adjust the space between LED and Pots to my liking, plus i didn't have to use any additional heatsink, as the LED were completely surrounded by air. | ||
For the first part I did not do controlled watering, i just gave them water by hand. | For the first part I did not do controlled watering, i just gave them water by hand. | ||
'''List of Parts''' | |||
*Housing made of MDF | |||
*Arduino Uno | |||
*2x 1W LED 660 nm (deep red) | |||
*2x 1W LED 440 nm (royal blue) | |||
*4x LDD-350L MEANWELL LED Driver | |||
*2x 12V Fan | |||
* generic switching relay | |||
*12V 4A power supply | |||
*terminal strip | |||
*Wires | |||
[[:File:artiPlants_01.zip|->Arduino Script]] | |||
[[File:day_0_box_web.jpg|390px|Front View of Growbox]] | [[File:day_0_box_web.jpg|390px|Front View of Growbox]] | ||
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As a first step i prepared thy seeds, by soaking them in warm water, which was boiled and thus sterilized before.<br/> | As a first step i prepared thy seeds, by soaking them in warm water, which was boiled and thus sterilized before.<br/> | ||
Then I prepared some Agar-Agar medium using the following recipe: | Then I prepared some Agar-Agar medium using the following recipe: | ||
'''Growth Medium Recipe'''<br/> | |||
''based on notes of Azucena Sánchez, adopted to what was available in the Lab'' | |||
*0,46 gr (2*0,23gr) Murishage Skoog medium (bought via ebay[https://www.ebay.de/usr/www_dephyte_com?_trksid=p2057872.m2749.l2754]) | |||
*4 gr (2*2gr) Sacharose | |||
*400ml (2*200ml) distilled water | |||
*0,96gr (2*0,48gr) agar | |||
stirred until nearly everything was dissolved<br/> | |||
pH medium 1 = 7,3 | pH medium 2 = 8,8 | |||
<gallery widths=120px heights=160px mode=nolines> | <gallery widths=120px heights=160px mode=nolines> |
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