Tardigrade (water bear): Difference between revisions

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→‎To grow them in the laboratory

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-'''In the laboratory''', we could take care of some of them. First we disolve the initial sample which had a huge amount of tardigrades and as food they had algae. Then some of them were "sacrifice" as a contaminated sample so we could observe them through the microscope. [[File:In the lab.png|thumb|disolving the sample]]At the begining they where looking full of food but after a month in this sample we found most of the tardigrades went to sleep mode probably due to lack of food.
+==To grow them in the laboratory==
+we needed to reproduce their living environment and feed them.
-Alessandro's input:
+We cultivated the specie ''Hypsibius exemplaris'' in a 25ml plastic bottle.
-We got clean tardigrade cultures of Hypsibius exemplaris and the algae on which they feed, Chloloroccum sp.
+We cultivated separately a culture of the alga "Chlorococcum" in a tube containing spring water (Volvic) and 0,01% of fertilizer (Algabloom). We kept in them in the tent for the algae, with 12h light exposure cycles.
-Each week we removed some water from the tardigrade flask, and add some water from the Chlorococcum flask. This way we feed them. The number of tardigrades in the culture increased significantly.
+Once a week, we fed the algae to the tardigrades as follows (each step needs a different sterile pipette tip):
-Chlorococcum was cultivated by refilling the missing water with spring water with 0,01% of plagron algal fertilizer.
+* Remove from the tardigrade culture half of the water on the top, without moving the biomass on the bottom
+* Replace the taken amount of water with the water coming from the Chlorococcus culture, aiming at taking the algae.
+* Refill the Chlorococcus culture with sterile cultivation medium (spring water with 0,01% of fertilizer algabloom)
+To observe them, with a sterile tip, take 200µl of water in the biomass at the bottom, put them on a slide and use the 10x magnification lens of the microscope to see them. [[File:In the lab.png|thumb|disolving the sample]]
-Then you can say which impression did you have while looking at it and learning about them