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== '''Which medium ??''' == | == '''Which medium ??''' == | ||
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http://www.instructables.com/id/Bioluminescent-Bacterial-Lightbulb-Water-Polluti/ | http://www.instructables.com/id/Bioluminescent-Bacterial-Lightbulb-Water-Polluti/ | ||
[[File:159756.jpg|400px]] | |||
===Step 1: What You'll Need=== | ===Step 1: What You'll Need=== | ||
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===Step 2: Get Your Hands on Some Dead Sea Fish=== | ===Step 2: Get Your Hands on Some Dead Sea Fish=== | ||
If you live by the ocean, GREAT. Just go down to the local fish market and get some fresh ( never frozen) squid, shirmp or fish from the sea. I tend to think squid is the best, then shrimp then sea fish since vibrio fischeri etc live inside the light organ of the bobtail squid, but there are tons of these guys all over the ocean. With a little luck the seafood you pick up will have the strains on it, if not, just try again or buy online like the rest of the land locked people. If you are land locked but visiting the beach you may be able to put the fish in a jar, then pack the jar inside a cooler with ice untill you get home but its not a guarntee. Or you can take some sterile agar culture plates with you and grow some bacteria while your on vacation and take it back home to your lab....your family may think your a bit strange but it's worth it ( I did it on a recent trip to Cali muwahahaha). | If you live by the ocean, GREAT. Just go down to the local fish market and get some fresh ( never frozen) squid, shirmp or fish from the sea. I tend to think squid is the best, then shrimp then sea fish since vibrio fischeri etc live inside the light organ of the bobtail squid, but there are tons of these guys all over the ocean. With a little luck the seafood you pick up will have the strains on it, if not, just try again or buy online like the rest of the land locked people. If you are land locked but visiting the beach you may be able to put the fish in a jar, then pack the jar inside a cooler with ice untill you get home but its not a guarntee. Or you can take some sterile agar culture plates with you and grow some bacteria while your on vacation and take it back home to your lab....your family may think your a bit strange but it's worth it ( I did it on a recent trip to Cali muwahahaha). | ||
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File:FMXEP06H4AGKBXN.LARGE.jpg|[http://www.instructables.com/id/Bioluminescent-Bacterial-Lightbulb-Water-Polluti/] | File:FMXEP06H4AGKBXN.LARGE.jpg|[http://www.instructables.com/id/Bioluminescent-Bacterial-Lightbulb-Water-Polluti/] | ||
</gallery> | </gallery> | ||
===Step 3: Make Some Bacteria Food Method # 1=== | ===Step 3: Make Some Bacteria Food Method # 1=== | ||
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<gallery> | <gallery> | ||
File:2220.jpg|7.December. it is not glowing :( | File:2220.jpg|7.December. it is not glowing :( | ||
File:5-01.jpg|14.Desember. they are growing but not glowing | File:5-01.jpg|14.Desember. they are growing but not glowing | ||
File:102.jpg|20.Desember. I use this bacteria to draw. I want to see how many details are visible when they are growing | File:102.jpg|20.Desember. I use this bacteria to draw. I want to see how many details are visible when they are growing | ||
</gallery> | </gallery> | ||
[[File:1546894.jpg|400px]] | |||
== The illuminated mushroom== | == The illuminated mushroom== | ||
This mushroom has developed an amazing feature, it shines in the dark. In our native forests sometimes infected by the Hallimasch root pieces. This phenomenon is referred to as bioluminescence and is also known by the fireflies. For most observers of yesteryear, the glowing wood was mysterious and eerie, and associated with wizards, fairies, and elves. In this mushroom species shine both the mycelium and the fruiting bodies. Interestingly, however, only the American style of Panellus stipticus shines. He has a creamy white color and 1-2 cm large, shell-like fruiting bodies that spread throughout the trunk. Every year new fruiting bodies appear. | This mushroom has developed an amazing feature, it shines in the dark. In our native forests sometimes infected by the Hallimasch root pieces. This phenomenon is referred to as bioluminescence and is also known by the fireflies. For most observers of yesteryear, the glowing wood was mysterious and eerie, and associated with wizards, fairies, and elves. In this mushroom species shine both the mycelium and the fruiting bodies. Interestingly, however, only the American style of Panellus stipticus shines. He has a creamy white color and 1-2 cm large, shell-like fruiting bodies that spread throughout the trunk. Every year new fruiting bodies appear. | ||
File: | [[File:15978.jpg|400px]]|Source: [https://www.plantura.garden/gartentipps/zierpflanzen/leuchtpilze-infos-bilder-anbau-anleitung] | ||
Nothing happens without reason in nature. So the extraordinary appearance of the mushrooms has a biological function. The emitted green light is visible to insects and attracts them. Similar to the "bees and flowers principle", the insects land on the mushrooms and transport on their onward flight spores that have stuck to them. Thus, the mushrooms receive by their striking luminous advantages in the propagation and distribution. | Nothing happens without reason in nature. So the extraordinary appearance of the mushrooms has a biological function. The emitted green light is visible to insects and attracts them. Similar to the "bees and flowers principle", the insects land on the mushrooms and transport on their onward flight spores that have stuck to them. Thus, the mushrooms receive by their striking luminous advantages in the propagation and distribution. | ||
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*temperature in the house should be all year round up to 25 ° C | *temperature in the house should be all year round up to 25 ° C | ||
I ordered the shining mushrooms from https://dikarbion.eu . I got it after a week. | |||
I first had to lay log in the water mixed with vinegar. It had to stay in there for 18 hours. | |||
Then I made the holes and put the mushrooms in. | |||
<gallery> | <gallery> | ||
File:2020.png|Source: [https://dikarbion.eu/epages/96a7356b-10eb-4075-b76d-a2feb5b9cba5.sf/de_DE/?ObjectPath=/Shops/96a7356b-10eb-4075-b76d-a2feb5b9cba5/Products/HabitatSetLeuchtpilz] | File:2020.png | ||
|Source: [https://dikarbion.eu/epages/96a7356b-10eb-4075-b76d-a2feb5b9cba5.sf/de_DE/?ObjectPath=/Shops/96a7356b-10eb-4075-b76d-a2feb5b9cba5/Products/HabitatSetLeuchtpilz] | |||
File:photo5442825236484565188.jpg | File:photo5442825236484565188.jpg | ||
File:235.JPG | |||
File:267.JPG | |||
File:159.JPG | |||
</gallery> | </gallery> | ||
[[File:596.JPG|400px]] | |||
Light fungus Finished culture in the WECK © Glas | Light fungus Finished culture in the WECK © Glas | ||
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</gallery> | </gallery> | ||
[[File:154689.jpg|400px]] | |||
File: | |||
== '''Modification of green fluorescent''' == | |||
I noticed that most artists used either bioluminecenses or fluresences bactera. | |||
But what's new: '''modified with fluorescent protein!''' | |||
I tried to know, what is it? and how can I do that? | |||
is a protein composed of 238 amino acid residues and shows a bright green fluorescence when exposed to blue to ultraviolet light. | |||
The artists have used it for art projects and scientists have used it to highlighting specific structures. | |||
[[File:GFP_Mice_01.jpg|400px]] [https://en.wikipedia.org/wiki/Green_fluorescent_protein#/media/File:GFP_Mice_01.jpg] | |||
Different proteins produce different fluorescent colors when exposed to ultraviolet light. (Picture taken by Erik A. Rodriguez.) | |||
https://en.wikipedia.org/wiki/Green_fluorescent_protein#/media/File:Fluorescence_from_Fluorescent_Proteins.jpg | |||
[[File:Fluorescence_from_Fluorescent_Proteins.jpg|400px]] | |||
[https://en.wikipedia.org/wiki/Green_fluorescent_protein#/media/File:Fluorescence_from_Fluorescent_Proteins.jpg] | |||
===Results=== | ===Results=== | ||
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File:calartsslide5-1.jpg[http://2012.igem.org/Team:Caltech/Human_Practice California Institute of the Arts to create animations from fluorescent bacteria] | File:calartsslide5-1.jpg[http://2012.igem.org/Team:Caltech/Human_Practice California Institute of the Arts to create animations from fluorescent bacteria] | ||
File:112.jpg|[https://www.huffingtonpost.com/entry/agar-art-contest_us_56253e13e4b02f6a900d3d57 composed of multiple petri dishes of bacteria modified with fluorescent protein under UV light] | File:112.jpg|[https://www.huffingtonpost.com/entry/agar-art-contest_us_56253e13e4b02f6a900d3d57 composed of multiple petri dishes of bacteria modified with fluorescent protein under UV light] | ||
File:e620b6f6238b6e05853d4dd3e848dc54--glow-in-dark-glow-in-the-dark-concrete.jpg|http://www.ohiotrm.org/glow-in-the-dark-table/glow-in-the-dark-table-with-photo-luminescent-resinyoutube/ | |||
File:images (2).jpg|http://www.ohiotrm.org/glow-in-the-dark-table/glow-in-the-dark-table-with-photo-luminescent-resinyoutube/ | |||
File:x-chromosome.jpg|https://www.huntercole.org/news/2017/10/12/donate-to-make-hunter-coles-art | |||
File:ABExploringbio-portrait.jpg|http://www.annebrodie.com/#/exploringtheinvisible/ | |||
File:hunter-cole-hands.jpg.838x0_q80.jpg|https://www.mnn.com/lifestyle/arts-culture/blogs/scientist-hunter-cole-bioluminescent-bacteria-creates-works-art | |||
File:CATERS_Solar_Powered_Bicycle_Path_06-800x498.jpg|https://www.catersnews.com/stories/quirky/watch-where-youre-glowing-artist-creates-worlds-first-solar-powered-cycle-path/ | |||
</gallery> | </gallery> | ||
Art / Design / Products using luminous bacteria | |||
*Simon Park - Exploring the invisible | |||
*Bioglyphs | |||
*Hunter Cole | |||
*Rebecca Klee | |||
*Kathy M. Takayama | |||
*Anne Brodie | |||
*Tim Otto Roth | |||
*Teresa Van Dongen | |||
*Marianne Engel | |||
*Thomas Feuerstein | |||
*Anna Dumitriu | |||
[https://hackteria.org/wiki/Explorations_in_BioLuminescence] | |||
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