295
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GMU:DIY Biolab “Driver’s License”/Mana Mazidy (view source)
Revision as of 13:44, 1 October 2018
, 1 October 2018→Glowing Banknote tell the true story.
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== '''Which medium ??''' == | == '''Which medium ??''' == | ||
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<gallery> | |||
[[File:af318a078ddef3c0c0b1d00c004293ff.jpg| | [[File:af318a078ddef3c0c0b1d00c004293ff.jpg|Source: [https://earlycanadianhistory.ca/2016/06/13/harriet-tubman-and-andrew-jackson-a-match-made-in-the-u-s-treasury-department/] | ||
</gallery> | |||
'''Ability''' | '''Ability''' | ||
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== BIOLUMINESCENT BACTERIAL LIGHTBULB== | |||
http://www.instructables.com/id/Bioluminescent-Bacterial-Lightbulb-Water-Polluti/ | http://www.instructables.com/id/Bioluminescent-Bacterial-Lightbulb-Water-Polluti/ | ||
[[File:159756.jpg|400px]] | |||
===Step 1: What You'll Need=== | |||
Fresh (but dead) Sea fish, squid or shrimp (if you don't have access to fresh fish you can simply buy the bacteria from Carolina biological supply here: http://www.carolina.com/product/vibrio+anguillarum%2C+living%2C+tube.do?keyword=Vibrio&sortby=bestMatches) | |||
Aquarium salt (from pet store) or fresh sea water | Aquarium salt (from pet store) or fresh sea water | ||
Agar growth medium ( dehyrated tryptic soy agar, or homemade, or ready to pour photobacterium agar...you get to choose if you want to make it or buy it.) | Agar growth medium ( dehyrated tryptic soy agar, or homemade, or ready to pour photobacterium agar...you get to choose if you want to make it or buy it.) | ||
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Distilled Water [http://www.instructables.com/id/Bioluminescent-Bacterial-Lightbulb-Water-Polluti/] | Distilled Water [http://www.instructables.com/id/Bioluminescent-Bacterial-Lightbulb-Water-Polluti/] | ||
===Step 2: Get Your Hands on Some Dead Sea Fish=== | |||
If you live by the ocean, GREAT. Just go down to the local fish market and get some fresh ( never frozen) squid, shirmp or fish from the sea. I tend to think squid is the best, then shrimp then sea fish since vibrio fischeri etc live inside the light organ of the bobtail squid, but there are tons of these guys all over the ocean. With a little luck the seafood you pick up will have the strains on it, if not, just try again or buy online like the rest of the land locked people. If you are land locked but visiting the beach you may be able to put the fish in a jar, then pack the jar inside a cooler with ice untill you get home but its not a guarntee. Or you can take some sterile agar culture plates with you and grow some bacteria while your on vacation and take it back home to your lab....your family may think your a bit strange but it's worth it ( I did it on a recent trip to Cali muwahahaha). | If you live by the ocean, GREAT. Just go down to the local fish market and get some fresh ( never frozen) squid, shirmp or fish from the sea. I tend to think squid is the best, then shrimp then sea fish since vibrio fischeri etc live inside the light organ of the bobtail squid, but there are tons of these guys all over the ocean. With a little luck the seafood you pick up will have the strains on it, if not, just try again or buy online like the rest of the land locked people. If you are land locked but visiting the beach you may be able to put the fish in a jar, then pack the jar inside a cooler with ice untill you get home but its not a guarntee. Or you can take some sterile agar culture plates with you and grow some bacteria while your on vacation and take it back home to your lab....your family may think your a bit strange but it's worth it ( I did it on a recent trip to Cali muwahahaha). | ||
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</gallery> | </gallery> | ||
===Step 3: Make Some Bacteria Food Method # 1=== | |||
Like you and me bacteria need food to grow so let's make some food that our bacteria will LOVE. Its full of vitamins, amino acids, carbon, minerals and of course salt, without salt they won't live (they live in the ocean after all). | Like you and me bacteria need food to grow so let's make some food that our bacteria will LOVE. Its full of vitamins, amino acids, carbon, minerals and of course salt, without salt they won't live (they live in the ocean after all). | ||
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Pour dissolved mixture of TSA, and Salt in a flask with a rag SLIGHTLY in the mouth and place in cooker. | Pour dissolved mixture of TSA, and Salt in a flask with a rag SLIGHTLY in the mouth and place in cooker. | ||
Place glass petri dishes (not needed if you bought some sterile plastic sealed ones) in as well. | |||
Fill cooker with about 1-2 inches of water. | |||
Seal pressure cooker and set heat to high | |||
When the top valve starts to dance and hiss start a timer for 15 min | |||
When time is up turn off heat and let cool down until pressure valve retracts | |||
Your agar gel and petri dishes are now completely sterilized !!! ( the steam molecules created under pressure bounces around and kills any organisms that were present) | Your agar gel and petri dishes are now completely sterilized !!! ( the steam molecules created under pressure bounces around and kills any organisms that were present) | ||
===Step 4: Bacteria Food Method # 2=== | |||
[[File:1030.jpg|400px]] [https://earlycanadianhistory.ca/2016/06/13/harriet-tubman-and-andrew-jackson-a-match-made-in-the-u-s-treasury-department/] | [[File:1030.jpg|400px]] [https://earlycanadianhistory.ca/2016/06/13/harriet-tubman-and-andrew-jackson-a-match-made-in-the-u-s-treasury-department/] | ||
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You will need: | You will need: | ||
Pancreatin (I used 325mg capsules) | *Pancreatin (I used 325mg capsules) | ||
Skim/Lowfat milk 7g | *Skim/Lowfat milk 7g | ||
100ml Distilled water | *100ml Distilled water | ||
Aquarium salt 3g | *Aquarium salt 3g | ||
Agar-Agar seaweed flakes 2g | *Agar-Agar seaweed flakes 2g | ||
Pour 7g of skim milk into a beaker. | Pour 7g of skim milk into a beaker. | ||
Add 100ml of distilled water and mix. Then add the contents of 1 pancreatin capsule and mix. | Add 100ml of distilled water and mix. Then add the contents of 1 pancreatin capsule and mix. | ||
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===Step 5: Bacteria Food Method # 3=== | |||
you can buy ready-to-pour "photobacterium" agar from Carolina Biological for about 7 bucks for 125ml....it's pricey but is the best for luminescent bacteria | |||
===Step 6: Let the Fish Glow=== | |||
[[File:1060.jpg|400px]] | [[File:1060.jpg|400px]] | ||
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I tryed to Make Some Bacteria Food : | I tryed to Make Some Bacteria Food : | ||
TSA agar medium | |||
NaCI 2g . | *NaCI 2g . | ||
Yeast Extract 1g . | *Yeast Extract 1g . | ||
Peptone 2g . | *Peptone 2g . | ||
Agar 3g . | *Agar 3g . | ||
Fill with H2O up till 200 ml | *Fill with H2O up till 200 ml | ||
== Photobacterium == | == Photobacterium == | ||
They are a genus of Gram-negative bacteria and belong to the family of Vibrionaceae. Some members of this genus have the ability to produce light and are therefore bioluminescent | |||
<gallery> | |||
File:2220.jpg|7.December. it is not glowing :( | |||
File:5-01.jpg|14.Desember. they are growing but not glowing | |||
File:102.jpg|20.Desember. I use this bacteria to draw. I want to see how many details are visible when they are growing | |||
</gallery> | |||
[[File:1546894.jpg|400px]] | |||
== The illuminated mushroom== | |||
This mushroom has developed an amazing feature, it shines in the dark. In our native forests sometimes infected by the Hallimasch root pieces. This phenomenon is referred to as bioluminescence and is also known by the fireflies. For most observers of yesteryear, the glowing wood was mysterious and eerie, and associated with wizards, fairies, and elves. In this mushroom species shine both the mycelium and the fruiting bodies. Interestingly, however, only the American style of Panellus stipticus shines. He has a creamy white color and 1-2 cm large, shell-like fruiting bodies that spread throughout the trunk. Every year new fruiting bodies appear. | |||
[[File:15978.jpg|400px]]|Source: [https://www.plantura.garden/gartentipps/zierpflanzen/leuchtpilze-infos-bilder-anbau-anleitung] | |||
Nothing happens without reason in nature. So the extraordinary appearance of the mushrooms has a biological function. The emitted green light is visible to insects and attracts them. Similar to the "bees and flowers principle", the insects land on the mushrooms and transport on their onward flight spores that have stuck to them. Thus, the mushrooms receive by their striking luminous advantages in the propagation and distribution. | Nothing happens without reason in nature. So the extraordinary appearance of the mushrooms has a biological function. The emitted green light is visible to insects and attracts them. Similar to the "bees and flowers principle", the insects land on the mushrooms and transport on their onward flight spores that have stuck to them. Thus, the mushrooms receive by their striking luminous advantages in the propagation and distribution. | ||
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*temperature in the house should be all year round up to 25 ° C | *temperature in the house should be all year round up to 25 ° C | ||
I ordered the shining mushrooms from https://dikarbion.eu . I got it after a week. | |||
I first had to lay log in the water mixed with vinegar. It had to stay in there for 18 hours. | |||
Then I made the holes and put the mushrooms in. | |||
<gallery> | <gallery> | ||
File:2020.png|Source: [https://dikarbion.eu/epages/96a7356b-10eb-4075-b76d-a2feb5b9cba5.sf/de_DE/?ObjectPath=/Shops/96a7356b-10eb-4075-b76d-a2feb5b9cba5/Products/HabitatSetLeuchtpilz] | File:2020.png | ||
|Source: [https://dikarbion.eu/epages/96a7356b-10eb-4075-b76d-a2feb5b9cba5.sf/de_DE/?ObjectPath=/Shops/96a7356b-10eb-4075-b76d-a2feb5b9cba5/Products/HabitatSetLeuchtpilz] | |||
File:photo5442825236484565188.jpg | File:photo5442825236484565188.jpg | ||
File:235.JPG | |||
File:267.JPG | |||
File:159.JPG | |||
</gallery> | </gallery> | ||
[[File:596.JPG|400px]] | |||
Light fungus Finished culture in the WECK © Glas | Light fungus Finished culture in the WECK © Glas | ||
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</gallery> | </gallery> | ||
[[File:154689.jpg|400px]] | |||
[[File: | |||
== '''Modification of green fluorescent''' == | == '''Modification of green fluorescent''' == | ||
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[[File:Fluorescence_from_Fluorescent_Proteins.jpg|400px]] | [[File:Fluorescence_from_Fluorescent_Proteins.jpg|400px]] | ||
[https://en.wikipedia.org/wiki/Green_fluorescent_protein#/media/File:Fluorescence_from_Fluorescent_Proteins.jpg] | [https://en.wikipedia.org/wiki/Green_fluorescent_protein#/media/File:Fluorescence_from_Fluorescent_Proteins.jpg] | ||
===Results=== | |||
The world is huge with so many hidden organism that are alive.This project let me to know this new world. helped me to learn how to control these little animals and learn the differences from them. | |||
Nothing happens without reason in nature. | |||
These little animals have helped me with their skills to explain my story. | |||
A co-operation to explain a collaboration between humans and nature to history. | |||
== Related projects== | |||
<gallery> | |||
File:1090.jpg|[https://www.uncommongoods.com/product/bioluminescent-aquarium | |||
Bioluminescent Aquarium] | |||
File:1080.jpg|[http://www.wired.co.uk/article/victoria-geaney-glowing-dress-bacteria This beautiful dress has a glowing secret: bacteria] | |||
File:1070.jpg|[https://www.newscientist.com/article/mg20827885-000-glowing-trees-could-light-up-city-streets/ Glowing trees could light up city streets] | |||
File:105060.png|[https://geneticliteracyproject.org/2017/04/10/glowing-trees-no-colonoscopies-wonders-synthetic-biology/ Glowing trees, no more colonoscopies and other wonders of synthetic biology] | |||
File:103060.jpg|[https://www.webinformational.com/glow-in-the-dark-bacteria-is-the-most-beautifully-unexpected-work-of-art-youll-ever-see/ This painting is a living thing–literally. Artists at the World Science Festival in Brisbane, Australia, are creating art from marine bacteria known as Aliivibrio fischeri.] | |||
File:100.jpg|[https://bscd.uchicago.edu/news/student-project-glowing-bacteria-art-gallery Student project of chicago university. fluoresced bacteria - meta-bacterial] | |||
File:MayanOnandOff_530_1.jpg|[http://flavorwire.com/398549/glow-in-the-dark-art-created-in-the-lab-with-bioluminescent-bacteria/view-all With Bioluminescent Bacteria - gallery at Montana State University] | |||
File:MILY.jpg|[http://www.miyaando.com/installations-1/2016/12/8/amwdv9kbdzbcliqsfs211iazo1pdiy from artist Miya Ando and photographer L. Young. With a different kind of bioluminescence] | |||
File:calartsslide5-1.jpg[http://2012.igem.org/Team:Caltech/Human_Practice California Institute of the Arts to create animations from fluorescent bacteria] | |||
File:112.jpg|[https://www.huffingtonpost.com/entry/agar-art-contest_us_56253e13e4b02f6a900d3d57 composed of multiple petri dishes of bacteria modified with fluorescent protein under UV light] | |||
File:e620b6f6238b6e05853d4dd3e848dc54--glow-in-dark-glow-in-the-dark-concrete.jpg|http://www.ohiotrm.org/glow-in-the-dark-table/glow-in-the-dark-table-with-photo-luminescent-resinyoutube/ | |||
File:images (2).jpg|http://www.ohiotrm.org/glow-in-the-dark-table/glow-in-the-dark-table-with-photo-luminescent-resinyoutube/ | |||
File:x-chromosome.jpg|https://www.huntercole.org/news/2017/10/12/donate-to-make-hunter-coles-art | |||
File:ABExploringbio-portrait.jpg|http://www.annebrodie.com/#/exploringtheinvisible/ | |||
File:hunter-cole-hands.jpg.838x0_q80.jpg|https://www.mnn.com/lifestyle/arts-culture/blogs/scientist-hunter-cole-bioluminescent-bacteria-creates-works-art | |||
File:CATERS_Solar_Powered_Bicycle_Path_06-800x498.jpg|https://www.catersnews.com/stories/quirky/watch-where-youre-glowing-artist-creates-worlds-first-solar-powered-cycle-path/ | |||
</gallery> | |||
Art / Design / Products using luminous bacteria | |||
*Simon Park - Exploring the invisible | |||
*Bioglyphs | |||
*Hunter Cole | |||
*Rebecca Klee | |||
*Kathy M. Takayama | |||
*Anne Brodie | |||
*Tim Otto Roth | |||
*Teresa Van Dongen | |||
*Marianne Engel | |||
*Thomas Feuerstein | |||
*Anna Dumitriu | |||
[https://hackteria.org/wiki/Explorations_in_BioLuminescence] | |||