GMU:DIY Biolab “Driver’s License”/Marie Laure Ebel: Difference between revisions

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-==WHO AM I? DNA ANALYSIS==
+==WHO SCIENTIFICALLY AM I/DNA ANALYSIS==
 I grew up with the fantasy that I must have something exotic deep inside me. People frequently asked me about my relatives, and I started to believe that I somehow belong to another soil than my motherland. Having the chance to work in a DIY Biolab, I decided to pursue my intersect feelings and I attended to analyze my DNA sequences, with the secret hope of discovering my own ethnicity extent and finding any connection with Asian pattern genome.
 <gallery>
 File:Blood collected2.jpg|Blood collected2
@@ Line 30: / Line 31: @@
 I sadly realized that I would never be able to find any sort of Asian connection in my blood, which brings me to present a project as a critic of this scientific supremacy. Current DNA analyses bring about an inaccurate truth and significant data are missing to determine one’s identity.
 That is why, I ended up to attempt to analyze my DNA and prove with primers that I am first a human, before being from a specific culture/nation/origin.
 ==PRACTICING PCR ANALYSIS==
 ===Step 1. Extracting DNA from blood and from pork liver meat===
+<gallery>
+File:STEP1.1.jpg
+File:STEP1.2.jpg
+File:STEP1.3.jpg
+File:STEP1.4.jpg
+File:STEP1.5.jpg
+File:STEP1.6.jpg
+File:STEP1.7.jpg
+File:STEP1.9.jpg
+File:STEP1.10.jpg
+File:STEP1.11.jpg
+File:STEP1.8.jpg
+</gallery>
-[[File:STEP1.1.jpg|170px]]
-[[File:STEP1.2.jpg|170px]]
-[[File:STEP1.3.jpg|170px]]
-[[File:STEP1.4.jpg|170px]]
-[[File:STEP1.5.jpg|170px]]
-[[File:STEP1.6.jpg|170px]]
-[[File:STEP1.7.jpg|170px]]
-[[File:STEP1.9.jpg|170px]]
-[[File:STEP1.10.jpg|170px]]
-[[File:STEP1.11.jpg|170px]]
-[[File:STEP1.8.jpg|170px]]
 ===Step 2. STR-analysis with pork meat primer===
 <gallery>
-File:STEP 2.1.jpg|STEP 2.1
+File:STEP 2.1.jpg
-File:STEP 2.2.jpg|STEP 2.2
+File:STEP 2.2.jpg
-File:STEP 2.3.jpg|STEP 2.3
+File:STEP2.3.jpg
-File:STEP 2.4.jpg|STEP 2.4
+File:STEP 2.4.jpg
-File:STEP 2.5.jpg|STEP 2.5
+File:STEP2.5.jpg
-File:STEP 2.6.jpg|STEP 2.6
+File:STEP 2.6.jpg
-File:STEP 2.7.jpg|STEP 2.7
+File:STEP 2.7.jpg
 </gallery>
 ===Step 3. Electrophoresis===
@@ Line 65: / Line 70: @@
 <gallery>
-File:STEP 3.1.JPG|STEP 3.1
+File:STEP 3.1.JPG
-File:STEP 3.2.JPG|STEP 3.2
+File:STEP3.2.JPG
-File:STEP 3.3.JPG|STEP 3.3
+File:STEP3.3.JPG
-File:STEP 3.4.jpg|STEP 3.4
+File:STEP 3.4.jpg
-File:STEP 3.5.jpg|STEP 3.5
+File:STEP 3.5.jpg
-File:STEP 3.6.jpg|STEP 3.6
+File:STEP 3.6.jpg
-File:STEP 3.7.JPG|STEP 3.7
+File:STEP 3.7.JPG
-File:STEP 3.8.JPG|STEP 3.8
+File:STEP3.8.JPG
-File:STEP 3.9.JPG|STEP 3.9
+File:STEP3.9.JPG
-File:STEP 3.10.JPG|STEP 3.10
+File:STEP3.10.JPG
 </gallery>
-=> I got no results: either the ladder nor the DNA samples were "growing" as they should (multiple lines).
+I got no results: either the ladder nor the DNA samples were "growing" as they should (multiple lines).
 So I decided to test the ladder only to see if there is a problem with the samples themselves or with the preparation (agarose + buffer TAE 50x+ distilled water) => #Second attempt
 ====#Second attempt====
 <gallery>
-File:STEP 3.2.1.jpg|STEP 3.2.1
+File:STEP 3.2.1.jpg
-File:STEP 3.2.2.jpg|STEP 3.2.2
+File:STEP 3.2.2.jpg
-File:STEP 3.2.3.jpg|STEP 3.2.3
+File:STEP 3.2.3.jpg
-File:STEP 3.2.4.jpg|STEP 3.2.5
+File:STEP 3.2.4.jpg
 </gallery>
-=> Same problem: the ladder was growing as one bloc only (see the little drawing). Even during a 2.2 attempt with new SERVA Stain G (which I thought it could be the problem since the previous tube was quite old). Then, I noticed that the positive side was turning in blue color and some part of the agarose gel was melting down.
+Same problem: the ladder was growing as one bloc only (see the little drawing). Even during a 2.2 attempt with new SERVA Stain G (which I thought it could be the problem since the previous tube was quite old). Then, I noticed that the positive side was turning in blue color and some part of the agarose gel was melting down.
 So I decided to test if this blue color is coming from oxidation of the electrodes themselves, or if the ladder was "swimming" on the extra amount of solution (buffer TAE 50x + distilled water) above the agarose to reach the positive side => #Third attempt
 ====#Third attempt====
+<gallery>
+File:STEP 3.3.1.jpg
+File:STEP 3.3.2.jpg
+</gallery>
-[[File:STEP 3.3.1.jpg|400px]]
+Still turning blue. Even with smaller electrodes that are completely covered by the solution (buffer TAE 50x + distilled water)
-[[File:STEP 3.3.2.jpg|400px]]
-=> Still turning blue. Even with smaller electrodes that are completely covered by the solution (buffer TAE 50x + distilled water)
 So I decided to test if this oxidation is coming from the buffer or if it is simply a natural process => #Fourth attempt
 ====#Fourth attempt====
+<gallery>
+File:STEP 3.4.1.jpg
+File:STEP 3.4.2.jpg
+</gallery>
-[[File:STEP 3.4.1.jpg|400px]]
+First picture: distilled water only. Second picture: distilled water + buffer TAE 50x. No changes at all.
-[[File:STEP 3.4.2.jpg|400px]]
-=> First picture: distilled water only. Second picture: distilled water + buffer TAE 50x. No changes at all.
 So it might come from the SERVA Stain G (which is yellow-ish) or it is actually not a problem and does not affect the electrophoresis itself.
+To resolve this problem, I have asked different professional/scientist for help in that matter. In addition, I am also searching for protocols which allow determining human DNA above animals/plants.
->> To resolve this problem, I have asked different professional/scientist for help in that matter. In addition, I am also searching for protocols which allow determining human DNA above animals/plants.
-References:
+==References==
-[https://isogg.org/wiki/Admixture_analyses#AncestryDNA_-_Genetic_Ethnicity]
+*https://isogg.org/wiki/Admixture_analyses#AncestryDNA_-_Genetic_Ethnicity
-[https://isogg.org/wiki/Autosomal_DNA#Autosomal_DNA_for_beginners]
+*https://isogg.org/wiki/Autosomal_DNA#Autosomal_DNA_for_beginners
-[http://www.sciencemag.org/news/2014/08/geneticists-decry-book-race-and-evolution]
+*http://www.sciencemag.org/news/2014/08/geneticists-decry-book-race-and-evolution
-[https://isogg.org/wiki/Genographic_Project]
+*https://isogg.org/wiki/Genographic_Project
-[https://en.wikipedia.org/wiki/Mitochondrial_DNA]
+*https://en.wikipedia.org/wiki/Mitochondrial_DNA
-[https://www.ucl.ac.uk/mace-lab/debunking/understanding]
+*https://www.ucl.ac.uk/mace-lab/debunking/understanding
-[http://synbio.info/display/synbio/Basics+of+Genetics]
+*http://synbio.info/display/synbio/Basics+of+Genetics
-[https://academic.oup.com/carcin/article/16/5/1243/305242]
+*https://academic.oup.com/carcin/article/16/5/1243/305242