295
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GMU:DIY Biolab “Driver’s License”/Mana Mazidy (view source)
Revision as of 13:33, 31 January 2018
, 31 January 2018→Bacteria
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[[File:FXZ0FQMH3Z32B68.LARGE.jpg|400px]] | [[File:FXZ0FQMH3Z32B68.LARGE.jpg|400px]][https://earlycanadianhistory.ca/2016/06/13/harriet-tubman-and-andrew-jackson-a-match-made-in-the-u-s-treasury-department/] | ||
If you live by the ocean, GREAT. Just go down to the local fish market and get some fresh ( never frozen) squid, shirmp or fish from the sea. I tend to think squid is the best, then shrimp then sea fish since vibrio fischeri etc live inside the light organ of the bobtail squid, but there are tons of these guys all over the ocean. With a little luck the seafood you pick up will have the strains on it, if not, just try again or buy online like the rest of the land locked people. If you are land locked but visiting the beach you may be able to put the fish in a jar, then pack the jar inside a cooler with ice untill you get home but its not a guarntee. Or you can take some sterile agar culture plates with you and grow some bacteria while your on vacation and take it back home to your lab....your family may think your a bit strange but it's worth it ( I did it on a recent trip to Cali muwahahaha). | If you live by the ocean, GREAT. Just go down to the local fish market and get some fresh ( never frozen) squid, shirmp or fish from the sea. I tend to think squid is the best, then shrimp then sea fish since vibrio fischeri etc live inside the light organ of the bobtail squid, but there are tons of these guys all over the ocean. With a little luck the seafood you pick up will have the strains on it, if not, just try again or buy online like the rest of the land locked people. If you are land locked but visiting the beach you may be able to put the fish in a jar, then pack the jar inside a cooler with ice untill you get home but its not a guarntee. Or you can take some sterile agar culture plates with you and grow some bacteria while your on vacation and take it back home to your lab....your family may think your a bit strange but it's worth it ( I did it on a recent trip to Cali muwahahaha). | ||
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'''Step 4: Bacteria Food Method # 2''' | '''Step 4: Bacteria Food Method # 2''' | ||
[[File:1030.jpg|400px]] | [[File:1030.jpg|400px]] [https://earlycanadianhistory.ca/2016/06/13/harriet-tubman-and-andrew-jackson-a-match-made-in-the-u-s-treasury-department/] | ||
A second method if you want to make bacteria food is to make your own nurtient agar from scratch (funner and cheaper). We will use PANCREATIN to digest the casein in milk into nutrients the bacteria can use and add some salt for minerals and agar-agar flakes to thicken it into a gel. | A second method if you want to make bacteria food is to make your own nurtient agar from scratch (funner and cheaper). We will use PANCREATIN to digest the casein in milk into nutrients the bacteria can use and add some salt for minerals and agar-agar flakes to thicken it into a gel. | ||