GMU:DIY Biolab “Driver’s License”/Mana Mazidy: Difference between revisions

GMU:DIY Biolab “Driver’s License”/Mana Mazidy (view source)

1,252 bytes added , 31 January 2018

295

edits

@@ Line 150: / Line 150: @@
 Pour still hot agar into sterile plates.
 Alow to cool into a gel and flip the plates over so gel is on the top.
+'''Step 5: Bacteria Food Method # 3
+'''you can buy ready-to-pour "photobacterium" agar from Carolina Biological for about 7 bucks for 125ml....it's pricey but is the best for luminescent bacteria
+'''Step 6: Let the Fish Glow'''
+Remove the squid from the bag and allow the ink to remain.
+Heat up your loop until it glows, allow it to cool for min, or use your sterile q-tip.
+Dip the loop/q-tip into the ink.
+Next streak the petri dish of bacteria food that you made with the loop or q-tip. (if you did good sterile technique, your dishes should not have any bacterial colonies growing on them when you streak it.)
+Streak the plates into 3-4 zones using an interrupted pattern as shown in the pic, this will help isolated colonies grow. Separate each streak with heating up your loop or using a new sterile q-tip. (The bacteria don't like to glow unless they are in isolated colonies, they are kinda shellfish uh I mean selfish)
+Place the plate in a cool dark (18-25 C) place and watch it grow and glow! I wrapped mine in newspaper to make sure they are in the dark.
+Note: Depending on temperature of the room and other factors, a newly streaked colony will take about 18-48 hours to grow large enought to turn on their glowing genes (lux genes).