GMU:DIY Biolab “Driver’s License”/Mana Mazidy: Difference between revisions

GMU:DIY Biolab “Driver’s License”/Mana Mazidy (view source)

Revision as of 13:11, 31 January 2018

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+'''Step 1: What You'll Need
+'''Fresh (but dead) Sea fish, squid or shrimp (if you don't have access to fresh fish you can simply buy the bacteria from Carolina biological supply here: http://www.carolina.com/product/vibrio+anguillarum%2C+living%2C+tube.do?keyword=Vibrio&sortby=bestMatches)
+Aquarium salt (from pet store) or fresh sea water
+Agar growth medium ( dehyrated tryptic soy agar, or homemade, or ready to pour photobacterium agar...you get to choose if you want to make it or buy it.)
+Sterile Petri Dishes (or glass bowls that can withstand being boiled)
+Sterile Q tip or loop of solid wire
+Pressure Cooker (optional but highly recomended)
+Large Beaker or saucepan
+Med size Erlenmeyer flask (optional)
+Distilled Water
-'''Get Your Hands on Some Dead Sea Fish'''
+'''Step 2: Get Your Hands on Some Dead Sea Fish
+'''
 http://www.instructables.com/id/Bioluminescent-Bacterial-Lightbulb-Water-Polluti/:
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+'''Step 3: Make Some Bacteria Food Method # 1
+'''
+Like you and me bacteria need food to grow so let's make some food that our bacteria will LOVE. Its full of vitamins, amino acids, carbon, minerals and of course salt, without salt they won't live (they live in the ocean after all).
+TSA agar gel recipe:
+Per Liter of deionized water: (you can always reduce it by 1/2 or 1/4 if you don't need the whole liter)
+g TSA agar (or directions per liter depending on which company makes it)
+g Salt
+Mix together in a large beaker or saucepan on a heating plate or stove.
+If you are going to use a pressure cooker you just have to heat it up until it mixes because using the pressure cooker to autoclave it will sterilize it...if you don't have a pressure cooker bring the mix to a steady controlled rolling boil to sterilize it.
+Pressure cooker instructions (wannabe autoclave)
+Pour dissolved mixture of TSA, and Salt in a flask with a rag SLIGHTLY in the mouth and place in cooker.
+ Place glass petri dishes (not needed if you bought some sterile plastic sealed ones) in as well.
+ Fill cooker with about 1-2 inches of water.
+ Seal pressure cooker and set heat to high
+ When the top valve starts to dance and hiss start a timer for 15 min
+ When time is up turn off heat and let cool down until pressure valve retracts
+Your agar gel and petri dishes are now completely sterilized !!! ( the steam molecules created under pressure bounces around and kills any organisms that were present)
+'''Step 4: Bacteria Food Method # 2'''
+A second method if you want to make bacteria food is to make your own nurtient agar from scratch (funner and cheaper). We will use PANCREATIN to digest the casein in milk into nutrients the bacteria can use and add some salt for minerals and agar-agar flakes to thicken it into a gel.
+Pancreatin can be found at your local vitamin store...it's used by people that have trouble digesting food due to problems with thier pancreas. The pancreas releases digestive enzymes. The pancreatin pills contain porcine (PIG!!!) amylase, lipase and protease that disolve protien, fat and carbs. OINK OINK.
+You will need:
+Pancreatin (I used 325mg capsules)
+Skim/Lowfat milk 7g
+ml Distilled water
+Aquarium salt 3g
+Agar-Agar seaweed flakes 2g
+Pour 7g of skim milk into a beaker.
+Add 100ml of distilled water and mix. Then add the contents of 1 pancreatin capsule and mix.
+Allow to sit for 8 hours with occasional stirring.
+Next add 3g of salt and 2g agar-agar flakes.
+Heat up mixture until salt and agar-agar disolves (maybe a SLOW boil, you don't want to burn your agar).
+If you have an autoclave er uh.... I mean pressure cooker, sterilize it for 15 min. as per previous instructions.
+Pour still hot agar into sterile plates.
+Alow to cool into a gel and flip the plates over so gel is on the top.
 == '''The illuminated mushroom''' ==