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GMU:DIY Biolab “Driver’s License”/Suna Yoo (view source)
Revision as of 16:34, 10 January 2018
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Azuzena showed us, what other people had already done. What stuck in my mind, was that you can heat mycelium and it will keep it´s form. For example in bricks or leather. | Azuzena showed us, what other people had already done. What stuck in my mind, was that you can heat mycelium and it will keep it´s form. For example in bricks or leather. | ||
Under the instructions of Azuzena i inoculated my first medium with spores. It was 15 minutes boiled cardboard. The container we used to store it, was ironically a blue supermarket Champignon box. After the Inoculation, wich in this case only means, to put a peace of wood with spores in it, between two or three sheets of hot cardboard. And then leave it in the oven, that is not called oven. | Under the instructions of Azuzena i inoculated my first medium with spores. It was 15 minutes boiled cardboard. The container we used to store it, was ironically a blue supermarket Champignon box. After the Inoculation, wich in this case only means, to put a peace of wood with spores in it, between two or three sheets of hot cardboard. And then leave it in the oven, that is not called oven. | ||
here will be a photo of the fungi I plucked | |||
4. 31.10. Halloween. Motivated by the experiences in the last lesson, i started doing something at home. This time i wanted to experiment with growing mycelium in coffee mold. I already had collected some for a time, because on my travels during the summer i had learned to use it instead of dish-soap. In addition i had plucked some fungi caps out of my garden, put them on a piece of paper and waited for them to drop their spores, facing their dead by drying out in my room. | 4. 31.10. Halloween. Motivated by the experiences in the last lesson, i started doing something at home. This time i wanted to experiment with growing mycelium in coffee mold. I already had collected some for a time, because on my travels during the summer i had learned to use it instead of dish-soap. In addition i had plucked some fungi caps out of my garden, put them on a piece of paper and waited for them to drop their spores, facing their dead by drying out in my room. | ||
So i took put on my new rollerskates ( | So i took put on my new rollerskates (3) and desinfected the whole kitchen with Korn (4), a typical thuringian alcohol. Then I boiled the coffee mold (5) for 15 minutes, maybe longer and put it in a bowl to let it cool down (6). Then i desinfected an old paprika sauce glass with Korn and put the boiled mold inside (7) rotatory with pieces of the dropped spores (8&9-11) and tapped it with kitchen paper. In the end i heated my oven to 50 degrees, let it cool down and then stored the glass in it with circulating air (12). Later that day i went to the lab to put in in the real machine. | ||
Here will be the pictures 3-12 | |||
5. Day. We started a Analization of our DNA. Means: gargle a sodium solution and then pipet a lot. I don´t know what and maybe even Miga does not really know. We follow the instruction. I think we seperated our DNA.. | 5. Day. We started a Analization of our DNA. Means: gargle a sodium solution and then pipet a lot. I don´t know what and maybe even Miga does not really know. We follow the instruction. I think we seperated our DNA.. | ||
This is a photo of my last experiment, 1 week later: | This is a photo of my last experiment, 1 week later: | ||
Photo | |||
I think it is contaminated, definetly too wet, says Miga. | I think it is contaminated, definetly too wet, says Miga. | ||
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Well i had a lot of worries. And yes not everything is really working how i thought, but i am still in the process. | Well i had a lot of worries. And yes not everything is really working how i thought, but i am still in the process. | ||
2 Photos of that Day | |||
9. 30.11.17 I met with Jan in the Lab, because he had a lot of PDA (Potatostarch-Dextrose-Agar) medium, he offered me to use for the first try. | 9. 30.11.17 I met with Jan in the Lab, because he had a lot of PDA (Potatostarch-Dextrose-Agar) medium, he offered me to use for the first try. | ||
I don´t have Photos of that Day, but here is, what came out a week later: | I don´t have Photos of that Day, but here is, what came out a week later: | ||
Photo | |||
It was already contaminated (the little greenish dots) and began to roll up (in the upper right corner). | It was already contaminated (the little greenish dots) and began to roll up (in the upper right corner). | ||