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GMU:DIY Biolab “Driver’s License”/DNA extraction (view source)
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Microvenus is a genetically modified Escherichia coli bacteria strain carrying a piece of synthetically composed sequence of amino acid molecules. It was first cloned into several laboratory strains of E. coli in collaboration with molecular geneticist Dana Boyd at Jon Beckwith’s laboratory at Harvard Medical School in 1988. As bacteria is small and invisible for the human eye, the artwork instead of being “aesthetic” is rather imaginable. Davis introduces the Microvenus as follow: | Microvenus is a genetically modified Escherichia coli bacteria strain carrying a piece of synthetically composed sequence of amino acid molecules. It was first cloned into several laboratory strains of E. coli in collaboration with molecular geneticist Dana Boyd at Jon Beckwith’s laboratory at Harvard Medical School in 1988. As bacteria is small and invisible for the human eye, the artwork instead of being “aesthetic” is rather imaginable. Davis introduces the Microvenus as follow: | ||
"Each Microvenus organism contains many copies of a special molecule designed by the artist and his colleagues. The artistic molecule is a short piece of synthetic DNA containing a coded visual icon that has been incorporated into a living strain of bacteria (E. Coli)." | "Each Microvenus organism contains many copies of a special molecule designed by the artist and his colleagues. The artistic molecule is a short piece of synthetic DNA containing a coded visual icon that has been incorporated into a living strain of bacteria (E. Coli)."* | ||
The coded icon is a symbol of life from a germanic rune. Resembling letter Y it also represents a female earth in different mythologies. The graphical image was converted into the bit-map image and further into DNA base pairs in order to synthetically program and attach to the original DNA code of the plasmids carried by bacteria (Fig. 6.). Davis has imagined that the code might be interpreted by extraterrestrials as the final stage of the project was supposed to include shipping of the modified bacteria into the universe. | The coded icon is a symbol of life from a germanic rune. Resembling letter Y it also represents a female earth in different mythologies. The graphical image was converted into the bit-map image and further into DNA base pairs in order to synthetically program and attach to the original DNA code of the plasmids carried by bacteria (Fig. 6.). Davis has imagined that the code might be interpreted by extraterrestrials as the final stage of the project was supposed to include shipping of the modified bacteria into the universe. | ||
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“The double-helix model of DNA structure was first published in the journal Nature by James Watson and Francis Crick in 1953”(https://en.wikipedia.org/wiki/Nucleic_acid_double_helix) | “The double-helix model of DNA structure was first published in the journal Nature by James Watson and Francis Crick in 1953”(https://en.wikipedia.org/wiki/Nucleic_acid_double_helix) | ||
<gallery> | |||
File:Wilson1900Fig2.jpg |Onion (Allium) cells in different phases of the cell cycle, drawn by E. B. Wilson, 1900 (wikipedia) | |||
File:Celltypes.png |A eukaryotic cell (left) and a prokaryotic cell (right) (wikipedia) | |||
File:DNA_Structure+Key+Labelled.pn_NoBB.png |The structure of the DNA double helix. The atoms in the structure are colour-coded by element and the detailed structures of two base pairs are shown in the bottom right.(wikipedia) | |||
File:1-dna-polymerase.jpg | |||
File:2-dna-structure.jpg | |||
File:3-what.jpg | |||
File:4-how.jpg | |||
</gallery> | |||
===PCR=== | ===PCR=== | ||
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==DNA extraction== | ==DNA extraction== | ||
https://www.youtube.com/watch?v=7wU97pTs-xE | |||
===Meat tissue cells=== | ===Meat tissue cells=== | ||
*mince thoroughly a small piece of meat tissue | *mince thoroughly a small piece of meat tissue | ||
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===Mouth cells=== | ===Mouth cells=== | ||
*50ml Falkon mit 50ml isotonischer Kochsalzlösung | *50ml Falkon mit 50ml [https://en.wikipedia.org/wiki/Saline_(medicine) isotonischer Kochsalzlösung] | ||
*damit den Mund ordentlich ausspülen (1-2min) | *damit den Mund ordentlich ausspülen (1-2min) | ||
*zurück in den Falkon spucken | *zurück in den Falkon spucken | ||