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→Workshop 2 (Gel electroforese chamber)
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==Workshop 2 (Gel electroforese chamber)== | ==Workshop 2 (Gel electroforese chamber)== | ||
*1. cutting out from plastic box | *1. cutting out from plastic box (Fig. 1.) | ||
*2. preparing screws | *2. preparing screws to be put into the chamber (Fig. 2.); screws will be connected later to the power supply blue (-12V) and yellow (+12V) cables | ||
*3. preparing 1% agarose (agarose, TAE, distilled water); for 100ml solution use 1g agarose, 2ml 50x TAE buffer and 98ml distilled water | *3. preparing 1% agarose (agarose, TAE, distilled water); for 100ml solution use 1g agarose, 2ml 50x TAE buffer and 98ml distilled water | ||
*4. pouring agarose solution into the chamber and adding SERVA DNA Stain G (5ml for 100ml solution) and mixing around so it spreads. | *4. pouring agarose solution into the chamber and adding SERVA DNA Stain G (5ml for 100ml solution) and mixing around so it spreads. | ||
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<gallery> | <gallery> | ||
File:pcr-IMG_4700.JPG | File:pcr-IMG_4700.JPG|Fig. 1 | ||
File:electrophoresis-IMG_4736.JPG | File:electrophoresis-IMG_4736.JPG|Fig. 2 | ||
File:electrophoresis-IMG_4741.JPG | File:electrophoresis-IMG_4741.JPG | ||
File:electrophoresis-IMG_4751.JPG | File:electrophoresis-IMG_4751.JPG | ||