Bureaucrats, emailconfirmed, Administrators
5,707
edits
| Line 116: | Line 116: | ||
| *1. cutting out from plastic box | *1. cutting out from plastic box | ||
| *2. preparing screws | *2. preparing screws | ||
| *3. preparing 1% agarose (agarose,  | *3. preparing 1% agarose (agarose, TAE, distilled water); for 100ml solution use 1g agarose, 2ml 50x TAE buffer and 98ml distilled water  | ||
| *4. pouring agarose solution into the chamber and adding SERVA DNA Stain G (5ml for 100ml solution) and mixing around so it spreads. | *4. pouring agarose solution into the chamber and adding SERVA DNA Stain G (5ml for 100ml solution) and mixing around so it spreads. | ||
| *5. cutting out endings of agarose for screws (screws get hot when letting electricity through and therefore it needs liquid outside) | *5. cutting out endings of agarose for screws (screws get hot when letting electricity through and therefore it needs liquid outside) | ||