GMU talk:Synthetic Biology/Bacteria Game/iGEM Wiki: Difference between revisions

GMU talk:Synthetic Biology/Bacteria Game/iGEM Wiki (view source)

Revision as of 15:35, 4 October 2010

166 bytes added , 4 October 2010

→‎Notebook

Lorenz

98

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 == Notebook ==
 === 29/10/2010 ===
-* [http://2010.igem.org/Transformation transformation] of TOP10 cells with plasmids out of the registry following [http://partsregistry.org/Help:Spring_2010_DNA_distribution#DNA_Kit_Plate_Instructions standard recommendations]
+* [http://2010.igem.org/Transformation transformation] of TOP10 cells with plasmids (see table 1) out of the registry following [http://partsregistry.org/Help:Spring_2010_DNA_distribution#DNA_Kit_Plate_Instructions standard recommendations]
 * over-night growth on selective agar plates
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 {| class="wikitable sortable" , border="1"
 |- bgcolor=grey
-! height=20px, align='center', width=80px | plasmid|| width=150px | Part || width=150px | pigment color || width=150 px | backbone || width=250 | registry location
+! height=20px, align='center', width=80px | plasmid|| width=150px | Part || width=150px | pigment color || width=150 px | backbone || width=150 | registry location
 |-style="height:20px"
 |+ align="bottom" |''<b>table 1:</b>'' overview of used iGEM constructs
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 |}
+<br/>
+* glycerol stocks (prepared directly after strains were received) from predator and prey cells (see table 2) were plated on selective agar plates
 <br/>
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 ! height=20px, align='center', width=80px | sample || width=150px | name/function || width=150px | cell strain || width=350 px | plasmid || width=100 | marker
 |-style="height:20px"
-|+ align="bottom" |''table 1:'' used predator-prey system, gift from R. Smith
+|+ align="bottom" |''table 2:'' used predator-prey system, gift from R. Smith
 |-align="center"
 | style="font-weight:bold;" | 1a || predator || MG1655  || ptetLuxRLasI-luxCcdA(SC101), placCcdBs-tetGFPuv(LVA) || Cm, Kan