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Since I already understood what my workflow is like, now I could create more precise plan. I wanted to film around 11 hours of timelapse (660 frames), so I'll stay in the BioLab almost the whole day. When I arrived at 6am I wanted to check two Petri dishes from my first experiment and then decide if I'll continue with them or create new ones. | Since I already understood what my workflow is like, now I could create more precise plan. I wanted to film around 11 hours of timelapse (660 frames), so I'll stay in the BioLab almost the whole day. When I arrived at 6am I wanted to check two Petri dishes from my first experiment and then decide if I'll continue with them or create new ones. | ||
In Petri dishes I noticed that slime mold changed color only in two spots: with blue and green colors, but blue one was the most visible. I assume, that place with red drop was hard ro reach for the slime mold (drops were on top of oat flakes), and place with the yellow one... I don't even know actually if it's natural color of the culture or not. But the first experiment was exactly for proving some of my concerns and to | In Petri dishes I noticed that slime mold changed color only in two spots: with blue and green colors, but blue one was the most visible. I assume, that place with red drop was hard ro reach for the slime mold (drops were on top of oat flakes), and place with the yellow one... I don't even know actually if it's natural color of the culture or not. But the first experiment was exactly for proving some of my concerns and to understand what to do next. | ||
I decided to prepare everything once again, because this material won't give me result that I want in the end. Also, my plan with using strong culture failed —there were mold inside. That's why I'll use slime mold from the first experiment.<gallery> | I decided to prepare everything once again, because this material won't give me result that I want in the end. Also, my plan with using strong culture failed —there were mold inside. That's why I'll use slime mold from the first experiment.<gallery> | ||
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A week after (18.05) I came to check all Petri dishes that I had. Red powder seems to be | A week after (18.05) I came to check all Petri dishes that I had. Red powder seems to be the best result, despite the fact that I have no idea what it is. <gallery mode="packed"> | ||
File:Exp 18.05 2.jpg | File:Exp 18.05 2.jpg | ||
File:Exp 18.05 3.jpg | File:Exp 18.05 3.jpg | ||
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</gallery>So I decided to buy a couple of Iron-oxide coloring powder and it should be delievered until 05.06. | </gallery>So I decided to buy a couple of Iron-oxide coloring powder and it should be delievered until 05.06. | ||
== | == Final Experiment (10.06 -13.06) == | ||
After I recieved 4 small packets with Iron-oxide coloring powder, I started new and last experiment. Firstly I prepared strong slime mold (03.06), and week after it was ready to use. In the same way, as before, I prepared agar, cooked | After I recieved 4 small packets with Iron-oxide coloring powder, I started new and last experiment. Firstly I prepared strong slime mold (03.06), and week after it was ready to use. In the same way, as before, I prepared agar, cooked poridge and then mixed it with coloring powder. Since I already tried red (unknown) powder before, this time I took 4 colors: white, green, blue and purple. Then I placed white and blue poridge in one Petri dish; green and purple in the second one.<gallery> | ||
File:Last coloring start.jpg|Prepared oatmeal for slime mold | File:Last coloring start.jpg|Prepared oatmeal for slime mold | ||
File:Last coloring ready.jpg|Oatmeal placed in Petri dishes with slime mold | File:Last coloring ready.jpg|Oatmeal placed in Petri dishes with slime mold | ||
</gallery> | </gallery>Three days after (13.06) I came to check the result. Slime mold grown up very well, but unfortunately it didn't change color at all. On pictures below it's clearly visible. Despite this fact, it was interesting to see that white color almost disappeared, and purple under microscope looks like with some sort of "sand/powder pattern". I have no idea whether this is the result of the dye having an uneven consistency on the oatmeal, or if there is another reason for it.<gallery mode="packed"> | ||
File:Last coloring done.jpg | File:Last coloring done.jpg | ||
File:Exp 13.06 1.jpg | File:Exp 13.06 1.jpg | ||
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File:Exp 13.06 15.jpg | File:Exp 13.06 15.jpg | ||
File:Exp 13.06 16.jpg | File:Exp 13.06 16.jpg | ||
</gallery> | </gallery>In any case, the slime mold essentially "disliked" this type of dye, and my attempts showed that an unidentified red powder from our laboratory yielded the best results. Due primarily to a lack of time for further testing, this experiment is the final one. | ||
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