GMU:DIY Bio: doing things with biology/Introduction to conceptual framework and tools
From Medien Wiki
Safety in the lab
- Goggles. Sometimes you will want to work with goggles. In case if something gets into the eyes: clean with running water!
- Gloves. While working with chemicals or need clean environment for your organisms, wear gloves!
- Started your work in the lab? Wash your hands!
- Finished the work in the lab? Wash your hands!
- No eating in the lab: you could contaminate your organisms/samples or, you could be poisoned by chemicals
- Apron/lab coat. It is better to use a lab coat
- Never pour chemicals back into the bottles
- Never put chemicals back into the jars
- Close containers immediately after pouring or taking out chemicals
- Do not inhale chemicals or contaminated plates
- Flame is not aloud. In case if needed to get rid of bacteria, be careful!
- Read instructions before using equipment which is not familiar
- Clean your work bench with 70% ethanol before and after the work
- Do not put your fingers into your eyes/mouth
Conceptual framework (References)
- File:Generation M .pdf Generation M Dimitris Papadopoulos
- File:Moholy-nagy.pdf The New Vision Laszlo Moholy-Nagy
- DIY Bio @ Hackteria, https://www.hackteria.org/
Growing microorganisms
Decision made to grow microorganisms from different surfaces. LBP (Baird-Parker liquid) recipe with sugars and proteins should be just fine for the test
LBP agar
- 1 liter water
- 1 g lactose
- 1 g peptone
- 5.1 g Na2HPO4∗7 H2O
- 4.2 g KH2PO4
- 20 g agar
References
Documentation
16.10.2018