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== | ==WHO SCIENTIFICALLY AM I/DNA ANALYSIS== | ||
I grew up with the fantasy that I must have something exotic deep inside me. People frequently asked me about my relatives, and I started to believe that I somehow belong to another soil than my motherland. Having the chance to work in a DIY Biolab, I decided to pursue my intersect feelings and I attended to analyze my DNA sequences, with the secret hope of discovering my own ethnicity extent and finding any connection with Asian pattern genome. | I grew up with the fantasy that I must have something exotic deep inside me. People frequently asked me about my relatives, and I started to believe that I somehow belong to another soil than my motherland. Having the chance to work in a DIY Biolab, I decided to pursue my intersect feelings and I attended to analyze my DNA sequences, with the secret hope of discovering my own ethnicity extent and finding any connection with Asian pattern genome. | ||
<gallery> | <gallery> | ||
File:Blood collected2.jpg|Blood collected2 | File:Blood collected2.jpg|Blood collected2 | ||
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</gallery> | </gallery> | ||
I sadly realized | I sadly realized that I would never be able to find any sort of Asian connection in my blood, which brings me to present a project as a critic of this scientific supremacy. Current DNA analyses bring about an inaccurate truth and significant data are missing to determine one’s identity. | ||
That is why, I ended up to attempt to analyze my DNA and prove with primers that I am first a human, before being from a specific culture/nation/origin. | |||
==PRACTICING PCR ANALYSIS== | ==PRACTICING PCR ANALYSIS== | ||
===Step 1. Extracting DNA from blood and from pork liver meat=== | ===Step 1. Extracting DNA from blood and from pork liver meat=== | ||
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File: | File:STEP1.1.jpg | ||
File: | File:STEP1.2.jpg | ||
File: | File:STEP1.3.jpg | ||
File: | File:STEP1.4.jpg | ||
File: | File:STEP1.5.jpg | ||
File: | File:STEP1.6.jpg | ||
File: | File:STEP1.7.jpg | ||
File: | File:STEP1.9.jpg | ||
File: | File:STEP1.10.jpg | ||
File: | File:STEP1.11.jpg | ||
File: | File:STEP1.8.jpg | ||
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File:STEP 2.1.jpg | File:STEP 2.1.jpg | ||
File:STEP 2.2.jpg | File:STEP 2.2.jpg | ||
File: | File:STEP2.3.jpg | ||
File:STEP 2.4.jpg | File:STEP 2.4.jpg | ||
File: | File:STEP2.5.jpg | ||
File:STEP 2.6.jpg | File:STEP 2.6.jpg | ||
File:STEP 2.7.jpg | File:STEP 2.7.jpg | ||
</gallery> | </gallery> | ||
===Step 3. Electrophoresis=== | ===Step 3. Electrophoresis=== | ||
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File:STEP 3.1.JPG | File:STEP 3.1.JPG | ||
File: | File:STEP3.2.JPG | ||
File: | File:STEP3.3.JPG | ||
File:STEP 3.4.jpg | File:STEP 3.4.jpg | ||
File:STEP 3.5.jpg | File:STEP 3.5.jpg | ||
File:STEP 3.6.jpg | File:STEP 3.6.jpg | ||
File:STEP 3.7.JPG | File:STEP 3.7.JPG | ||
File: | File:STEP3.8.JPG | ||
File: | File:STEP3.9.JPG | ||
File: | File:STEP3.10.JPG | ||
</gallery> | </gallery> | ||
I got no results: either the ladder nor the DNA samples were "growing" as they should (multiple lines). | |||
So I decided to test the ladder only to see if there is a problem with the samples themselves or with the preparation (agarose + buffer TAE 50x+ distilled water) => #Second attempt | So I decided to test the ladder only to see if there is a problem with the samples themselves or with the preparation (agarose + buffer TAE 50x+ distilled water) => #Second attempt | ||
====#Second attempt==== | ====#Second attempt==== | ||
<gallery> | <gallery> | ||
File:STEP 3.2.1.jpg | File:STEP 3.2.1.jpg | ||
File:STEP 3.2.2.jpg | File:STEP 3.2.2.jpg | ||
File:STEP 3.2.3.jpg | File:STEP 3.2.3.jpg | ||
File:STEP 3.2.4.jpg | File:STEP 3.2.4.jpg | ||
</gallery> | </gallery> | ||
Same problem: the ladder was growing as one bloc only (see the little drawing). Even during a 2.2 attempt with new SERVA Stain G (which I thought it could be the problem since the previous tube was quite old). Then, I noticed that the positive side was turning in blue color and some part of the agarose gel was melting down. | |||
So I decided to test if this blue color is coming from oxidation of the electrodes themselves, or if the ladder was "swimming" on the extra amount of solution (buffer TAE 50x + distilled water) above the agarose to reach the positive side => #Third attempt | So I decided to test if this blue color is coming from oxidation of the electrodes themselves, or if the ladder was "swimming" on the extra amount of solution (buffer TAE 50x + distilled water) above the agarose to reach the positive side => #Third attempt | ||
====#Third attempt==== | ====#Third attempt==== | ||
<gallery> | |||
File:STEP 3.3.1.jpg | |||
File:STEP 3.3.2.jpg | |||
</gallery> | |||
Still turning blue. Even with smaller electrodes that are completely covered by the solution (buffer TAE 50x + distilled water) | |||
So I decided to test if this oxidation is coming from the buffer or if it is simply a natural process => #Fourth attempt | So I decided to test if this oxidation is coming from the buffer or if it is simply a natural process => #Fourth attempt | ||
====#Fourth attempt==== | ====#Fourth attempt==== | ||
<gallery> | |||
File:STEP 3.4.1.jpg | |||
File:STEP 3.4.2.jpg | |||
</gallery> | |||
First picture: distilled water only. Second picture: distilled water + buffer TAE 50x. No changes at all. | |||
So it might come from the SERVA Stain G (which is yellow-ish) or it is actually not a problem and does not affect the electrophoresis itself. | |||
To resolve this problem, I have asked different professional/scientist for help in that matter. In addition, I am also searching for protocols which allow determining human DNA above animals/plants. | |||
==References== | |||
*https://isogg.org/wiki/Admixture_analyses#AncestryDNA_-_Genetic_Ethnicity | |||
*https://isogg.org/wiki/Autosomal_DNA#Autosomal_DNA_for_beginners | |||
*http://www.sciencemag.org/news/2014/08/geneticists-decry-book-race-and-evolution | |||
*https://isogg.org/wiki/Genographic_Project | |||
*https://en.wikipedia.org/wiki/Mitochondrial_DNA | |||
*https://www.ucl.ac.uk/mace-lab/debunking/understanding | |||
*http://synbio.info/display/synbio/Basics+of+Genetics | |||
*https://academic.oup.com/carcin/article/16/5/1243/305242 |