CULTIVATION OF Haematococcus Pluvialis.
- Known by its red color, it's an alga that in stress conditions produces a high content of a strong antioxidant known as astaxanthin.
* SCIENTIFIC CLASIFICATION Division: Chlorophyta Class: Chlorophyceae Order: Chlamydomonadales Family: Haematococcaceae Genus: Haematococcus Species: H. pluvialis Binomial name Haematococcus pluvialis
Sequence of growth states of the Haematococcus: https://comunicarciencia.bsm.upf.edu/?tag=haematococcus-pluvialis
- 100ml of water
- 3% of vinasse
- 0.7% of NaCl.
The pH is adjusted to 7.0. A 0.4 g/L quantity of inoculum can be used for the initial culture (cells in vegetative growth). The culture must be performed with 0.5 vvm air at 25°C, and until 15 days of culture.
Note: in the laboratory was no Vinasse, so another bioproduct of the sugar was used in order to prepare the medium Malz extract. (brennwert: 1.216kJ, fett 0.2g, kohlenhydrate 65g, Zucker 45g, Eiweiss 5g, Salz0.02g.)
The three samples contain:
1 Sample 3,00g malz extract, 100ml water & 0,7g NACl. 2 Sample 0,30g malz extract, 100ml water & 0,7g NACl. 3 Sample 0,03g malz extract, 100ml water & 0,7g NACl.
From left to right more concentration of vinasse and less according to the made samples.
The sample if full with specimens, that can be seen with the eye like a red spot. Under the microscope, some of the specimens are starting to acquire a green border.
In the sample, I found another specimen, that is probably feating from the Haematococcus pluvialis
In the last sample, the concentration of haematococcus can't be seen by the eye, but under the microscope the specimen can be seen as shown in the pictures.
Conclusions: the environmental conditions for the Haematococcus-pluvialis are not ideal, in order for it to get out of it hibernation state. My first hypothesis is that it doesn't have the ideal conditions in terms of light and temperature, and my second hypothesis is that it doesn't have the ideal medium (a vinasse medium wasn't used for the growth of the specimen, instead a similar medium was made).