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'''CULTIVATION OF ''Haematococcus Pluvialis''. ''' | '''CULTIVATION OF ''Haematococcus Pluvialis''. ''' | ||
- | - Known by its red color, it's an alga that in stress conditions produces a high content of a strong antioxidant known as astaxanthin. | ||
''* SCIENTIFIC CLASIFICATION'' | |||
Division: Chlorophyta | |||
Class: Chlorophyceae | |||
Order: Chlamydomonadales | |||
Family: Haematococcaceae | |||
Genus: Haematococcus | |||
Species: H. pluvialis | |||
Binomial name Haematococcus pluvialis | |||
MEDIUM | ''https://en.wikipedia.org/wiki/Haematococcus_pluvialis'' | ||
''https://upload.wikimedia.org/wikipedia/commons/4/48/Mikrofoto.de-Blutregenalgen_3.jpg'' | |||
[[File:HCP 1.PNG|400px]] | |||
Sequence of growth states of the Haematococcus: | |||
''https://comunicarciencia.bsm.upf.edu/?tag=haematococcus-pluvialis'' | |||
* MEDIUM | |||
- 100ml of water | - 100ml of water | ||
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- 0.7% of NaCl. | - 0.7% of NaCl. | ||
The pH is adjusted to 7.0. A 0.4 g/L quantity of inoculum can be used for the initial culture (cells in vegetative growth). The culture must be performed with 0.5 vvm air at 25°C, and until 15 days of culture. | |||
Note: in the laboratory was no Vinasse, so another bioproduct of the sugar was used in order to prepare the medium ''Malz extract''. | Note: in the laboratory was no Vinasse, so another bioproduct of the sugar was used in order to prepare the medium ''Malz extract''. | ||
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* Day 2: An observation under the microscope is made in order to see the specimen. | * Day 2: An observation under the microscope is made in order to see the specimen. | ||
((Ask for the photos... made day 3)) | |||
* Day 4: After 4 days of growth the specimens don't show any change of state. At the moment it would be expected that they start to acquire an orange color ''(if the conditions were ideal for the specimen)''. A hypothesis is that the medium is not the one that they need in order to survive, or that the conditions of light and warm are not ideal for them. | |||
[[File:wd9.JPG|400px]] | |||
* Day 14: After 10 days the Haematococcus is settling in the bottom of the tube, it didn't acquire any other color, what it means that it conditions were not ideal for them to develop. | |||
[[File:wd10.JPG|400px]] | |||
A new medium for the specimen must be done, and further investigation has also to be done, in order to find the ideal conditions to cultivate it and for it to grow. (For the moment the specimen is in a hibernation state. |
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