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- Medium: | - Medium: | ||
*100ml of distilled water | |||
100ml of distilled water | *2g of agar | ||
*Leaflets | |||
2g of agar | |||
Leaflets | |||
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- Medium: | - Medium: | ||
*NaCl 30 g | |||
NaCl 30 g | *Glycerol 1 g | ||
*Pepton (Bacxto-peptone) 10 g | |||
Glycerol 1 g | *Meat extract 3 g | ||
*Made up with distilled water to 1000 ml | |||
Pepton (Bacxto-peptone) 10 g | |||
Meat extract 3 g | |||
Made up with distilled water to 1000 ml | |||
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* Day 4: I failed to extract the ''Alii Vibrio Fischeri'' into the medium, after three days there was no blue glowing as seen in the first day inside the medium that I made. My hypothesis is that the specimen wasn't in the same original conditions from where it comes from, and it couldn´t survive. | * Day 4: I failed to extract the ''Alii Vibrio Fischeri'' into the medium, after three days there was no blue glowing as seen in the first day inside the medium that I made. My hypothesis is that the specimen wasn't in the same original conditions from where it comes from, and it couldn´t survive. | ||
CONCLUSIONS: Because I failed in the extraction of the Aliivibrio Fischeri, I will start with the cultivation of ''Polycephalum/physarum'' in order to confirm if this specimen can be cultivated in salty environments. According to the previous investigations of Dussutour, it is possible, so I will explore this idea. | CONCLUSIONS: Because I failed in the extraction of the Aliivibrio Fischeri, I will start with the cultivation of ''Polycephalum/physarum'' in order to confirm if this specimen can be cultivated in salty environments. According to the previous investigations of Dussutour, it is possible, so I will explore this idea. | ||