1) During the time that I adopted this specimen, I learned about its ideal medium of surviving, and also how it reacts according to its environmental conditions. During this time a medium was created in order to see the specimen reaction and to take the specimen out of its hibernation state(because of lack of vinasse, a supplement was used in the cooking of the medium).
After the attempt of cultivation of this specimen, I get into the conclusion that the environmental conditions for the Haematococcus-pluvialis were not ideal, in order for it to get out of its hibernation state. My first hypothesis is that it didn't have the ideal conditions in terms of light, air, and temperature, and my second hypothesis is that it didn't have the ideal medium (lack of vinasse in the medium).
- 100ml of water - 3% of vinasse - 0.7% of NaCl.
The pH is adjusted to 7.0. A 0.4 g/L quantity of inoculum can be used for the initial culture (cells in vegetative growth). The culture must be performed with 0.5 vvm air at 25°C, and until 15 days of culture.
* SCIENTIFIC CLASIFICATION
Division: Chlorophyta Class: Chlorophyceae Order: Chlamydomonadales Family: Haematococcaceae Genus: Haematococcus Species: H. pluvialis Binomial name Haematococcus pluvialis
Sequence of growth states of the Haematococcus: https://comunicarciencia.bsm.upf.edu/?tag=haematococcus-pluvialis Known by its red color, it's an alga that in stress conditions produces a high content of a strong antioxidant known as astaxanthin.
Diary of work.
Here can find the diary of work around the experiments that I made with the specimen H.P. Haematococcus Pluvialis.
- Day 1: Three samples were made all with the same concentration of salinity but with different percentage of vinasse, and with the same PH 7. Preparation and from left to right; original medium, 10% of original medium diluted in 100ml of water, 1% of original sample diluted in 100ml of water.
Note: in the laboratory was no Vinasse, so another bioproduct of the sugar was used in order to prepare the medium Malz extract. (brennwert: 1.216kJ, fett 0.2g, kohlenhydrate 65g, Zucker 45g, Eiweiss 5g, Salz0.02g.)
The three samples contain:
1 Sample 3,00g malz extract, 100ml water & 0,7g NACl. 2 Sample 0,30g malz extract, 100ml water & 0,7g NACl. 3 Sample 0,03g malz extract, 100ml water & 0,7g NACl.
- Day 2: An observation under the microscope is made in order to see the specimen.
- Day 4: After 4 days of growth the specimens haven't acquired an orange color (this color was expected if the conditions of the environment were ideal for the specimen).
- Day 10: The specimen seems to be starting to attach to the bottom of the tube.
- Day 15: I made an observation of the three samples under the microscope, here are the results:
From left to right more concentration of vinasse and less according to the made samples.
- 1 Sample 3,00g malz extract, 100ml water & 0,7g NACl.
The sample if full with specimens, that can be seen with the eye like a red spot. Under the microscope.
- 2 Sample 0,30g malz extract, 100ml water & 0,7g NACl.
In the sample, I found another specimen, that is probably feating from the Haematococcus pluvialis
- 3 Sample 0,03g malz extract, 100ml water & 0,7g NACl.
In the last sample, the concentration of haematococcus can't be seen by the eye, but under the microscope, the specimen can be seen as shown in the pictures, also some of the specimens are starting to acquire a green border.''