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''' ''Polycephalum/physarum'' ''' | ''' ''Polycephalum/physarum'' ''' | ||
- Wikipedia link: https:// | - Wikipedia link: https://en.wikipedia.org/wiki/Physarum_polycephalum ''(general information around this specimen)'' | ||
- TED: https://www.ted.com/speakers/heather_barnett_1 ''(a conference opening a window around experiments with this specimen)'' | - TED: https://www.ted.com/speakers/heather_barnett_1 ''(a conference opening a window around experiments with this specimen)'' | ||
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'''EXPERIMENTATION with Alii Vibrio Fischeri''' | '''EXPERIMENTATION with Alii Vibrio Fischeri''' | ||
* Day 1: The extraction of ''Alii Vibrio Fischeri'' from a living natural environment ''(took from an octopus)''. | * Day 1: The extraction of ''Alii Vibrio Fischeri'' from a living natural environment ''(took from an octopus)''. The process consists in taking a sample from the living specimen where the shining in the dark can be seen and then taking a piece of the meet where this specimen lives and putting them all inside of the medium. The sample has to be in a place with 20°C of temperature and in darkness. | ||
[[File:wd11.JPG|400px]] | [[File:wd11.JPG|400px]] | ||
* Day 4: I failed to extract the ''Alii Vibrio Fischeri'' into the medium, after three days there was no blue glowing as seen in the first day inside the medium that I made. My hypothesis is that the specimen wasn't in the same original conditions from where it comes from, and it couldn´t survive. | |||
CONCLUSIONS: Because I failed in the extraction of the Aliivibrio Fischeri, I will start with the cultivation of ''Polycephalum/physarum'' in order to confirm if this specimen can be cultivated in salty environments, and more specifically if it can survive in a 3% concentration of NaCl medium. According to the previous investigations of Dussutour, it is possible for it to survive in salinity environments, so I will explore this idea. | |||
'''EXPERIMENTATION with ''Polycephalum/physarum'' ''' | '''EXPERIMENTATION with ''Polycephalum/physarum'' ''' | ||
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[[File:wd12.JPG|400px]] | [[File:wd12.JPG|400px]] | ||
After 5 days the samples of ''Polycephalum/physarum'' | After 5 days the samples of ''Polycephalum/physarum'' didn't came to live again, my first hypothesis is that the medium was to salty my second is that the specimen needs more time in order to come to live again (awake from hibernation state). | ||
[[File:wd23.jpg|400px]] | [[File:wd23.jpg|400px]] | ||
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[[File:wd35.jpg|400px]] | [[File:wd35.jpg|400px]] | ||
*Day 12: Definetly the samples in the salinity medium are | *Day 12: Definetly the samples in the salinity medium are death. Ant the contamination/chemical reaction continues in the other samples. | ||
[[File:wd36.jpg|400px]] | [[File:wd36.jpg|400px]] | ||
* Day 15: Because the salinity in the medium is to high for the specimens to growth, I reduced the salinity inside the medium into .3% in order to see the reaction of the specimen. The right samples continues to be just a control sample with an ideal medium and the medium sample | * Day 15: Because the salinity in the medium is to high for the specimens to growth, I reduced the salinity inside the medium into .3% in order to see the reaction of the specimen. The right samples continues to be just a control sample with an ideal medium and the medium in the middle is a medium in between with food in the none salinity medium. | ||
[[File:wd37.jpg|400px]] | |||
* Day 17: For the ideal medium the specimen is growing, but after a week it acquire some mode, the middle sample move to the food area away from the salinity medium, and the specimen in the salinity medium started to move in order to try to find food. It is tolerating the salinity medium. The specimen in the salinity medium with 0.3% concentration of NaCl is starting to get into hibernation mode, I put some food in order to make it strong before transplanting it into a new medium with higher concentrations of NaCl. | |||
[[File:wd38.jpg|400px]] | |||
*Day 19: I made an observation under the microscope, the pink color in my samples is a bacteria, contamination that comes from the first petri dish that I used to extract a clean sample. Here can be found more pictures of the contamination. | |||
Because of health conditions and the use that can be given in the laboratory where I work, I put away this samples (disposed of them in a clean way). | |||
<gallery> | |||
File:wd39.jpg | |||
File:wd40.jpg | |||
File:wd41.jpg | |||
File:wd42.jpg | |||
File:wd43.jpg | |||
File:wd45.jpg | |||
</gallery> | |||
*Day 20: I continue increasing the salinity quantities in the medium of the specimen to 0.4% and 0.5%. It is working. | |||
Because of the vacation period, I put all my samples into rest (hibernation) and I will wake them up again in 3 weeks. | |||
File:wd46.jpg | |||
File:wd47.jpg | |||
*Day 45: |
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