GMU:Flagelates, Nematodes, and I/Sarah Horn

Research and Ideas

What fascinates me most about Euglena gracilis is its ability to use photosynthesis or rather the need of this capability to survive. Photosynthesis means that the organism converts light energy into chemical energy to power its energy balance. Euglena subsist heterotrophic and autotrophic; besides the process of photosynthesis (which is used by plants) it takes nourishment like animals as well. Euglena gracilis is a mixotrophic microorganism. Besides that, it is able to recognize the stimulus of light (phototaxis). This function helps Euglena to move towards light sources to always assure the facility to use photosynthesis. [1]

During my researches I read an article about the project of a former student. She did a couple of experiments which included a tube wrapped with red foil to create a red light source. The result was that the organisms in the tube piled up on the only transparent place without foil because they don't recognize red light.

This made me think of several questions:

• What happens when the whole tube is wrapped in foil and there is only red light? What will Euglena's concentration be like?
• How does Euglenas react to other colors?
• Is there a color they prefer?
• Can I create contrasts in the concentration by using different colors?

Furthermore, I wondered if it is possible to work creatively with Euglena’s phototaxis. As we can see, it always moves towards the light, so how about taking control over that?

• If I cover a glass receptacle with a dark medium, let’s say black, nontransparent paper, and cut out a shape to let light in, will the organisms accumulate there and fill this shape?
• Is it possible to arrange Euglenas in forms I want them to be?

Preparation

First of all I cooked a medium for Euglena which should promote the reproduction. It contains specific nutrients. Combined with a light position, e.g. at the window or in front of a lamp, they should accrete fast. Those microorganisms cannot be seen with the naked eye but if the population increases, the medium becomes greenish. This is my target goal because that makes it easier to work with.

Experiments

Experiment 1

Setup: I filled a tube with the Euglena medium (capacity: 15ml) and added about ten drops of Euglenas. To make the light colorful I cut out three stripes of transparent paper and wrapped them around the tube. I chose the fundamental colors yellow, red and blue.

Expectation: I guess that the concentration will be very low behind the red and blue foil. As I read during my researches, Euglenas don’t react to red light. Blue is a pretty dark color and I think it won’t attract the organisms much. The yellow paper seems to be the most transparent, so my assumption is that the concentrations will be the highest there.

Outcome: