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In the following video which i uploaded to Youtube you can see the Nematodes through a cellphone microscope. At thst time, hoewever, they haven't moved yet.: | In the following video which i uploaded to Youtube you can see the Nematodes through a cellphone microscope. At thst time, hoewever, they haven't moved yet.: | ||
[https://youtu.be/qBg0eJOhR_U Nematodes that don't move] | [https://youtu.be/qBg0eJOhR_U Nematodes that don't move] | ||
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Revision as of 07:45, 13 January 2021
Nematodes, do they prefer sugar or salt?
Project
I am trying to find out how nematode Heterorhabditis bacteriophora are reacting to salt and sugar. I'm trying to find out if they prefer one environment more or even die in one. The nematodes stay in petri dishes with water, as we have found out that this is where they have the best chance of survival
General informations about nematodes:
[[1]] (Wikipedia)
-> tiny, threadlike, colorless, unsegmented roundworms -> over 40.000 species (many are not yet figured out) -> not related to earthworms -> plant-feeding nematodes live in soil -> most of them are to small the be seen with naked eye but the longest known nematode is thirteen meters long and it resides in the sperm whale
first attempts to cook a medium in the laboratory:
Project implementation idea:
I try to find nematodes in soil by dissolving the earth in water I am going to try to divide the namdeaths found in the earth into 4 petri dishes.
the petri dishes contain the following:
first petri dish: Nematodes in water (as a control, as they should survive here for a while)
second petri dish: Nematodes and salt which is dissolved in water
third petri dish: Nematodes and sugar which is dissolved in water
fourth petri dish: Nematodes and sugar on the on and salt on the other side of the petri dish with a little bit of water (to see if they prefer and move to one side)
My first attempts to find Nematodes in soil:
The Microscope i am using
The soil i have tried to disolve in water
The image you can see looking through the microscope with a smartphone camera
-> unfortuantely i can see no Nematodes
It is very hard to find Nematodes in soil and especially afterwards to isolate them.
Because of that Miga is going to send me Nematodes to my post adress at home.
Day 1 with the new Nematodes
Today i received the Nematodes. I am going to disolve them in water (room temperature) and try to bring them back to life. As soon as they start to move i am going to contiune the expermient.
Remark: If you are trying find nematodes in soil and isolate them afterwards as i tried in the first place you may find this website helpful: Nematode Isolation
In the following video which i uploaded to Youtube you can see the Nematodes through a cellphone microscope. At thst time, hoewever, they haven't moved yet.: Nematodes that don't move