Marie EBEL (talk | contribs) No edit summary |
Marie EBEL (talk | contribs) No edit summary |
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=> I got no results : either the ladder nor the DNA samples were "growing" as they should (multiple lines). | => I got no results : either the ladder nor the DNA samples were "growing" as they should (multiple lines). | ||
So I decided to test the ladder only to see if there is a problem from the samples themselves or with the preparation (agarose + buffer + distilled water) => #Second attempt | So I decided to test the ladder only to see if there is a problem from the samples themselves or with the preparation (agarose + buffer TAE 50x+ distilled water) => #Second attempt | ||
#Second attempt | #Second attempt | ||
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=> Same problem : the ladder was growing as one bloc only (see the little drawing) + we noticed that the positive side was turning in blue color and some part of the agarose gel was melting down. | => Same problem : the ladder was growing as one bloc only (see the little drawing) + we noticed that the positive side was turning in blue color and some part of the agarose gel was melting down. | ||
So we decided to | So we decided to test if this bleu color is coming from oxidation of the electrodes themselves, or if the ladder was "swimming" on the extra amount of solution (buffer TAE 50x + distilled water) above the agarose to reach the positive side => #Third attempt | ||
#Third attempt | |||