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== '''Which medium ??''' == | == '''Which medium ??''' == | ||
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http://www.instructables.com/id/Bioluminescent-Bacterial-Lightbulb-Water-Polluti/ | http://www.instructables.com/id/Bioluminescent-Bacterial-Lightbulb-Water-Polluti/ | ||
[[File:159756.jpg|400px]] | |||
===Step 1: What You'll Need=== | ===Step 1: What You'll Need=== | ||
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===Step 2: Get Your Hands on Some Dead Sea Fish=== | ===Step 2: Get Your Hands on Some Dead Sea Fish=== | ||
If you live by the ocean, GREAT. Just go down to the local fish market and get some fresh ( never frozen) squid, shirmp or fish from the sea. I tend to think squid is the best, then shrimp then sea fish since vibrio fischeri etc live inside the light organ of the bobtail squid, but there are tons of these guys all over the ocean. With a little luck the seafood you pick up will have the strains on it, if not, just try again or buy online like the rest of the land locked people. If you are land locked but visiting the beach you may be able to put the fish in a jar, then pack the jar inside a cooler with ice untill you get home but its not a guarntee. Or you can take some sterile agar culture plates with you and grow some bacteria while your on vacation and take it back home to your lab....your family may think your a bit strange but it's worth it ( I did it on a recent trip to Cali muwahahaha). | If you live by the ocean, GREAT. Just go down to the local fish market and get some fresh ( never frozen) squid, shirmp or fish from the sea. I tend to think squid is the best, then shrimp then sea fish since vibrio fischeri etc live inside the light organ of the bobtail squid, but there are tons of these guys all over the ocean. With a little luck the seafood you pick up will have the strains on it, if not, just try again or buy online like the rest of the land locked people. If you are land locked but visiting the beach you may be able to put the fish in a jar, then pack the jar inside a cooler with ice untill you get home but its not a guarntee. Or you can take some sterile agar culture plates with you and grow some bacteria while your on vacation and take it back home to your lab....your family may think your a bit strange but it's worth it ( I did it on a recent trip to Cali muwahahaha). | ||
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File:FMXEP06H4AGKBXN.LARGE.jpg|[http://www.instructables.com/id/Bioluminescent-Bacterial-Lightbulb-Water-Polluti/] | File:FMXEP06H4AGKBXN.LARGE.jpg|[http://www.instructables.com/id/Bioluminescent-Bacterial-Lightbulb-Water-Polluti/] | ||
</gallery> | </gallery> | ||
===Step 3: Make Some Bacteria Food Method # 1=== | ===Step 3: Make Some Bacteria Food Method # 1=== | ||
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File:102.jpg|20.Desember. I use this bacteria to draw. I want to see how many details are visible when they are growing | File:102.jpg|20.Desember. I use this bacteria to draw. I want to see how many details are visible when they are growing | ||
</gallery> | </gallery> | ||
[[File:1546894.jpg|400px]] | |||
== The illuminated mushroom== | == The illuminated mushroom== | ||
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File:|Source: [https://www.plantura.garden/gartentipps/zierpflanzen/leuchtpilze-infos-bilder-anbau-anleitung] | [[File:15978.jpg|400px]]|Source: [https://www.plantura.garden/gartentipps/zierpflanzen/leuchtpilze-infos-bilder-anbau-anleitung] | ||
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</gallery> | </gallery> | ||
[[File:154689.jpg|400px]] | |||
== '''Modification of green fluorescent''' == | |||
I noticed that most artists used either bioluminecenses or fluresences bactera. | |||
But what's new: '''modified with fluorescent protein!''' | |||
I tried to know, what is it? and how can I do that? | |||
is a protein composed of 238 amino acid residues and shows a bright green fluorescence when exposed to blue to ultraviolet light. | |||
The artists have used it for art projects and scientists have used it to highlighting specific structures. | |||
[[File:GFP_Mice_01.jpg|400px]] [https://en.wikipedia.org/wiki/Green_fluorescent_protein#/media/File:GFP_Mice_01.jpg] | |||
Different proteins produce different fluorescent colors when exposed to ultraviolet light. (Picture taken by Erik A. Rodriguez.) | |||
https://en.wikipedia.org/wiki/Green_fluorescent_protein#/media/File:Fluorescence_from_Fluorescent_Proteins.jpg | |||
[[File:Fluorescence_from_Fluorescent_Proteins.jpg|400px]] | |||
File: | [https://en.wikipedia.org/wiki/Green_fluorescent_protein#/media/File:Fluorescence_from_Fluorescent_Proteins.jpg] | ||
===Results=== | ===Results=== | ||
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File:ABExploringbio-portrait.jpg|http://www.annebrodie.com/#/exploringtheinvisible/ | File:ABExploringbio-portrait.jpg|http://www.annebrodie.com/#/exploringtheinvisible/ | ||
File:hunter-cole-hands.jpg.838x0_q80.jpg|https://www.mnn.com/lifestyle/arts-culture/blogs/scientist-hunter-cole-bioluminescent-bacteria-creates-works-art | File:hunter-cole-hands.jpg.838x0_q80.jpg|https://www.mnn.com/lifestyle/arts-culture/blogs/scientist-hunter-cole-bioluminescent-bacteria-creates-works-art | ||
File:CATERS_Solar_Powered_Bicycle_Path_06-800x498.jpg|https://www.catersnews.com/stories/quirky/watch-where-youre-glowing-artist-creates-worlds-first-solar-powered-cycle-path/ | |||
</gallery> | </gallery> | ||
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[https://hackteria.org/wiki/Explorations_in_BioLuminescence] | [https://hackteria.org/wiki/Explorations_in_BioLuminescence] | ||
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