GMU:DIY Biolab “Driver’s License”/Jan Georg Glöckner/The supermarket cloning project: Difference between revisions
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Abstract | '''Abstract''' | ||
Specimen of mushrooms obtained from weimerian supermarkets are cloned and purified, than stored in a cold-storage strain-bank. | |||
Via reverse-engineering I extract the strand from the product sold. The strands will be available at an exhibition. | |||
'''Methodology''' | |||
Seven dishes per strand. PDA- medium. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. | |||
The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, it is inserted in the hole that the hot scalpel made. | |||
The dishes are labeled and incubated at room temperature (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till clean and strong. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strand, enveloped in a zip-loc-bag. | |||
Revision as of 16:21, 28 January 2018
Abstract
Specimen of mushrooms obtained from weimerian supermarkets are cloned and purified, than stored in a cold-storage strain-bank. Via reverse-engineering I extract the strand from the product sold. The strands will be available at an exhibition.
Methodology
Seven dishes per strand. PDA- medium. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, it is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated at room temperature (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till clean and strong. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strand, enveloped in a zip-loc-bag.