Jan Glöckner (talk | contribs) No edit summary |
Jan Glöckner (talk | contribs) No edit summary |
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killed several Euglena with the help of other students. | killed several Euglena with the help of other students. | ||
we where supposed to cook LA-Medium. | we where supposed to cook LA-Medium. | ||
cause of death: | |||
-lacking of vital ingredients | -lacking of vital ingredients | ||
-too much vitamin B12 | -too much vitamin B12 | ||
- ph-level was 3,6 (should have been 4,6) we messed it when we tried to adjust ph with hydrochloric acid, because of the dropper giving way to big droplets) | - ph-level was 3,6 (should have been 4,6) we messed it when we tried to adjust ph with hydrochloric acid, because of the dropper giving way to big droplets) | ||
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Pleurotus grow is, if, growing very slowly, planning to move on of the two pegs to another medium. | Pleurotus grow is, if, growing very slowly, planning to move on of the two pegs to another medium. | ||
supplementary: vibrio phosp. opened jar A and | supplementary: vibrio phosp. opened jar A and shock it vigorously under the laminar hood, to get oxygen to the bacteria, which should aid glowing. no effect. the sample has to be discarded anyway. | ||
'''9. November''' | '''9. November''' | ||
Revision as of 23:50, 16 November 2017
The digital lab-book
Starting in mid-october
killed several Euglena with the help of other students. we where supposed to cook LA-Medium. cause of death:
-lacking of vital ingredients
-too much vitamin B12
- ph-level was 3,6 (should have been 4,6) we messed it when we tried to adjust ph with hydrochloric acid, because of the dropper giving way to big droplets)
25. October
inoculated cooked cardboard with Pleurotus pegs.
31. October
starting to grow vibrio phosphoreum from a batch I obtained from Miga. They were labelled Photobacterium phosp. and seem to be inoculated somewhere in 2016. (the writing on the petri-dish was rendered unreadable)
cooked 100ml of LA-Medium and left it overnight in the fridge.
on plastic petri-dishes: I decided to use only glass-petri-dishes, as they give me the opportunity to sterilise medium inside the dishes, thus limiting to chances for contamination greatly. and they produce less waste, are to be recycled if broken, if not broken have a unlimited shelf life and usage time.
migrated the pleurotus from the plastic mushroom supermarket dish to a tupperware. no sign of growth.
1.November
Autoclaved two dishes filled with LA-Medium, one of the dishes filled up with water during the process, discarded and poured with leftover medium that has been in the autoclave, and inoculated with vibrio phosphoreum around 16.00. they should start to glow in 12-24 hours.
2.November
Pleurotus growth becomes apparent. vibrio phosp. still no luminescence. maybe the concentration of the the autoinducer is still to low in the medium. (Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 42 subchapt. Auto induction)
Euglena seem to spin when there is not enough light for them, rather an otherwise. Which makes perfect sense (appetitive behaviour) but somehow I thought it to be the other way round.
8. November
vibrio phosp. dish A shows signs of contamination, I assume due to autoclaving problems as mentioned above. still no luminescence, I am beginning to suspect them to be dark mutants (Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 43 subchapt. Formation of dark mutants)
Pleurotus grow is, if, growing very slowly, planning to move on of the two pegs to another medium.
supplementary: vibrio phosp. opened jar A and shock it vigorously under the laminar hood, to get oxygen to the bacteria, which should aid glowing. no effect. the sample has to be discarded anyway.
9. November
Discarded jar A of vibrio phosp. due to high contamination. transferred on of the pleurotus pegs on malt medium. (Paul Stamets, The Mushroom Cultivator)
10. November The incubator was switched of. Switched it back on again.
15. November
Pleurotus in malt-medium spawns cottony mycelium. The sample shows mild contamination. the tupperware sample still is, if growing very slowly but shows no signs of contamination. the temperature of the incubator is now 25.5
16.November Cooked and sterilised 1 Liter of Potato-Dextrose-Agar-Medium. Poured 5 petri-dishes, to be sterilised on friday. Still no luminescence in vibr. phosp.