GMU:DIY Biolab “Driver’s License”/Jan Georg Glöckner

The digital lab-book

Starting in mid-october

killed several Euglena with the help of other students. we where supposed to cook LA-Medium. cute of death: -lacking of vital ingredients -too much vitamin B12 - ph-level was 3,6 (should have been 4,6) we messed it when we tried to adjust ph with hydrochloric acid, because of the dropper giving way to big droplets)

25. October

inoculated cooked cardboard with Pleurotus pegs.

31. October

starting to grow vibrio phosphoreum from a batch I obtained from Miga. They were labelled Photobacterium phosp. and seem to be inoculated somewhere in 2016. (the writing on the petri-dish was rendered unreadable)

cooked 100ml of LA-Medium and left it overnight in the fridge.

on plastic petri-dishes: I decided to use only glass-petri-dishes, as they give me the opportunity to sterilise medium inside the dishes, thus limiting to chances for contamination greatly. and they produce less waste, are to be recycled if broken, if not broken have a unlimited shelf life and usage time.

migrated the pleurotus from the plastic mushroom supermarket dish to a tupperware. no sign of growth.

1.November

Autoclaved two dishes filled with LA-Medium, one of the dishes filled up with water during the process, discarded and poured with leftover medium that has been in the autoclave, and inoculated with vibrio phosphoreum around 16.00. they should start to glow in 12-24 hours.

2.November

Pleurotus growth becomes apparent. vibrio phosp. still no luminescence. maybe the concentration of the the autoinducer is still to low in the medium. (Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 42 subchapt. Autoinduction)