GMU:DIY Biolab “Driver’s License”/Jan Georg Glöckner: Difference between revisions

Latest revision as of 15:56, 31 March 2018

The digital lab-book ( this is the unedited version of the lab-book, that will be the most up-to-date document)

supermarket piracy programm

Oyster mushroom cloning series stage 1 (obtained from Globus supermarket; polish origin)

Brown Button mushroom cloning series stage 1,2 and stage 3 [petri dishes A to D and G.H not shown] ( obtained from Rewe supermarket; german origin)

Artistic Abstract

To address the growing issue of patented living organisms, I pirate the genome of store-bought mushroom-products, by reverse engineering the product to its viable state of mycelium. I then distribute the organism, for everyone to culture and distribute, in a respectful way and to form mutual diplomatic relations. As the genetic makeup of the strains change through cultivation, the creative commons licence is applied to the genome to keep it from being obscured by companies.

CC BY-SA 4.0

Scientific Abstract

Specimen of mushrooms obtained from supermarkets are cloned and the developing strain is purified, than stored in a cold-storage strain-bank. Via reverse-engineering the strain from a commercial product is extracted and cultured. The strains will be given away, and kept available.

Method

For every attempt of cloning at least seven petri-dishes filled with PDA- medium are inoculated. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, inside the stem or just under the cap. It is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated in the incubator (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till there is no visible evidence of contaminants. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strain, enveloped in a zip-loc-bag. ( liquid suspended-animation technique will be introduced.)

References

Paul Stamets and J.S. Chilton: The Mushroom Cultivator; Sterile Technique and Agar Culture: Starting a Culture From Live Tissue (p. 29) 1983 Agarikon Press Olympia Washington ISBN: 978-0-9610798-0-2
Paul Stamets: Growing Gourmet and Medicinal Mushrooms (Third Edition); Culturing Mushroom Mycelium on Agar Media: Starting a Mushroom Strain by Cloning (p. 88); 2000 Ten Speed Press ( Random House New York) ISBN: 978-1-58008-175-7

@@ Line 1: / Line 1: @@
-'''The digital lab-book'''
+[[/The digital lab-book|The digital lab-book]] ( this is the unedited version of the lab-book, that will be the most up-to-date document)
-'''Starting in mid-october'''
-killed several Euglena with the help of other students.
+[[/General methods|General methods]]
-we where supposed to cook LA-Medium.
-cute of death:
--lacking of vital ingredients
--too much vitamin B12
-- ph-level was 3,6 (should have been 4,6) we messed it when we tried to adjust ph with hydrochloric acid, because of the dropper giving way to big droplets)
-'''25. October'''
-inoculated cooked cardboard with Pleurotus pegs.
+==supermarket piracy programm==
-'''31. October'''
+Oyster mushroom cloning series stage 1 (obtained from Globus supermarket; polish origin)
+<gallery>
+File:Pleur.ostr.SAD1.JPG|
+File:Pleur.ostr.SAD1 alternateview.JPG|
+File:Pleur.ostr.SAD2.JPG|
+File:Pleur.ostr.SAD3.JPG|
+File:Pleur.ostr.SAD4.JPG|
+File:Pleur.ostr.SBD1.JPG|
+File:Pleur.ostr.SBD2.JPG|
+File:Pleur.ostr.SBD3.JPG|
+File:Pleur.ostr.SBD3 alternateview.JPG|
+</gallery>
-starting to grow vibrio phosphoreum from a batch I obtained from Miga. They were labelled Photobacterium phosp. and seem to be inoculated somewhere in 2016. (the writing on the petri-dish was rendered unreadable)
+Brown Button mushroom cloning series stage 1,2 and stage 3 [petri dishes A to D and G.H not shown] ( obtained from Rewe supermarket; german origin)
-cooked 100ml of LA-Medium and left it overnight in the fridge.
+<gallery>
+File:button E1 alternateview.JPG
+File:button E2.JPG
+File:button E3.JPG
+</gallery>
-''on plastic petri-dishes: I decided to use only glass-petri-dishes, as they give me the opportunity to sterilise medium inside the dishes, thus limiting to chances for contamination  greatly. and they produce less waste, are to be recycled if broken, if not broken have a unlimited shelf life and usage time.''
-migrated the pleurotus from the plastic mushroom supermarket dish to a tupperware. no sign of growth.
+===Artistic Abstract===
-'''1.November'''
+To address the growing issue of patented living organisms, I pirate the genome of store-bought mushroom-products, by reverse engineering the product to its viable state of mycelium. I then distribute the organism, for everyone to culture and distribute, in a respectful way and to form mutual diplomatic relations. As the genetic makeup of the strains change through cultivation, the creative commons licence is applied to the genome to keep it from being obscured by companies.
-Autoclaved two dishes filled with LA-Medium, one of the dishes filled up with water during the process, discarded and poured with leftover medium that has been in the autoclave, and inoculated with vibrio phosphoreum around 16.00.
-they should start to glow in 12-24 hours.
-'''2.November'''
+[https://creativecommons.org/licenses/by-sa/4.0/ CC BY-SA 4.0]
-Pleurotus growth becomes apparent.
+===Scientific Abstract===
-vibrio phosp. still no luminescence. maybe the concentration of the the autoinducer is still to low in the medium. ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 42 subchapt. Auto induction)''
-Euglena seem to spin when there is not enough light for them, rather an otherwise. Which makes perfect sense (appetitive behaviour) but somehow I thought it to be the other way round.
+Specimen of mushrooms obtained from supermarkets are cloned and the developing strain is purified, than stored in a cold-storage strain-bank. Via reverse-engineering the strain from a commercial product is extracted and cultured. The strains will be given away, and kept available.
-'''8. November'''
+===Method===
-vibrio phosp. dish A shows signs of contamination, I assume due to autoclaving problems as mentioned above.
+For every attempt of cloning at least seven petri-dishes filled with PDA- medium are inoculated. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, inside the stem or just under the cap. It is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated in the incubator (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till there is no visible evidence of contaminants. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strain, enveloped in a zip-loc-bag. ( liquid suspended-animation technique will be introduced.)
-still no luminescence, I am beginning to suspect them to be dark mutants ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 43 subchapt. Formation of dark mutants)''
-Pleurotus grow is, if, growing very slowly, planning to move on of the two pegs to another medium.
+===References===
-supplementary: vibrio phosp. opened jar A and shocked it vigorously under the laminar hood, to get oxygen to the bacteria, which should aid glowing. no effects. the sample has to be discarded anyway.
+*Paul Stamets and J.S. Chilton: The Mushroom Cultivator; Sterile Technique and Agar Culture: Starting a Culture From Live Tissue (p. 29) 1983 Agarikon Press Olympia Washington ISBN: 978-0-9610798-0-2
+*Paul Stamets: Growing Gourmet and Medicinal Mushrooms (Third Edition); Culturing Mushroom Mycelium on Agar Media: Starting a Mushroom Strain by Cloning (p. 88); 2000 Ten Speed Press ( Random House New York) ISBN: 978-1-58008-175-7
-'''9. November'''
-Discarded jar A of vibrio phosp. due to high contamination.
-transferred on of the pleurotus pegs on malt medium. (Paul Stamets, The Mushroom Cultivator)
-'''10. November'''
-The incubator was switched of. Switched it back on again.
-'''15. November'''
-Pleurotus in malt-medium spawns cottony mycelium. The sample shows mild contamination.
-the tupperware sample still is, if growing very slowly but shows no signs of contamination.
-the temperature of the incubator is now 25.5
-.November
-Cooked and sterilised 1 Liter of Potato-Dextrose-Agar-Medium. Poured 5 Petri dishes, to be sterilised on friday.