GMU:DIY Bio: doing things with biology/Sirin Unmanee/30.10.2018 : Isolating Bacteria from Kombucha, Growing Physarum polycephalum (2nd attempt) and others: Difference between revisions
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<br />''27.11.2018 - 18:30'' | <br />''27.11.2018 - 18:30'' | ||
Then I detached them again, but I added food coloring in the petri dishes, Yellow medium for Yellow bacteria and Orange medium for Orange bacteria. | Then I detached them again, but I added food coloring in the petri dishes, Yellow medium for Yellow bacteria and Orange medium for Orange bacteria. Moreover, in this time I changed the medium to the LBP (Baird-Parker liquid) medium. | ||
LBP agar | |||
<br />1 liter water | |||
<br />1 g lactose | |||
<br />1 g peptone | |||
<br />5.1 g Na2HPO4∗7 H2O | |||
<br />4.2 g KH2PO4 | |||
<br />20 g agar | |||
[[File:S__3571892.jpg|200px]] [[File:S__3571893.jpg|200px]] | [[File:S__3571892.jpg|200px]] [[File:S__3571893.jpg|200px]] | ||
<br />''27.11.2018 - 19:30'' | <br />''27.11.2018 - 19:30'' | ||
After one week, the shape was different, it was more separated than when I cultivated them with Gluconcetobacter medium. | |||
[[File:S__3694594.jpg|200px]][[File:S__3694595.jpg|200px]] | |||
<br />''04.12.2018 - 19:30'' | |||
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In conclusion, Kombucha bacteria can be growing on both types of medium, with Gluconcetobacter medium they will form a shape like a colony, but with LBP medium they are more apart. | |||
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<br />''09.11.2018 - 13:30'' | <br />''09.11.2018 - 13:30'' | ||
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After two weeks, they died (again) due to the unsuitable temperature. It's more difficult to grow the Physarum during winter. | After two weeks, they died (again) due to the unsuitable temperature. It's more difficult to grow the Physarum during winter. | ||
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[[File:S__3284996.jpg|200px]] | [[File:S__3284996.jpg|200px]] | ||
<br />''07.11.2018 - 10:30'' | <br />''07.11.2018 - 10:30'' | ||
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They are contaminated, so I took one of the bacteria which is not fully contaminated to other dish. | They are contaminated, so I took one of the bacteria which is not fully contaminated to other dish. | ||
Revision as of 08:46, 5 December 2018
Isolating Bacteria from Kombucha
Here is list of ingredients to prepare 500 ml of liquid Gluconcetobacter medium
Glucose - 10 g
Peptone - 2.5 g
Yeast extract - 2.5 g
Na2HPO4 - 1.35g
Citric acid - 0.75 g
Distilled or tap water - 500 ml
Agar - 7.5 g
However, in this case, I needed just 100 ml, so everything was divided by 5.
I diluted 2 ml of Kombucha and 8 ml of distilled water, then smeared one drop of that solution onto the first slot. The others' solutions were diluted in 1/2, 1/4, 1/8, 1/16 and 1/32 ratio respectively.
30.10.2018 - 20:30
There are yellow and orange dots in 1/4 and 1/32 slot, but others remain almost the same, except the medium is covered with icy surface.
07.11.2018 - 10:30
07.11.2018 - 10:30
I detached Orange and Yellow bacteria into other petri dishes.
20.11.2018 - 19:30
I found some interesting patterns here.
After one week, I detached the Yellow and Orange bacteria again. They formed a different shape this time.
27.11.2018 - 18:30
Then I detached them again, but I added food coloring in the petri dishes, Yellow medium for Yellow bacteria and Orange medium for Orange bacteria. Moreover, in this time I changed the medium to the LBP (Baird-Parker liquid) medium.
LBP agar
1 liter water
1 g lactose
1 g peptone
5.1 g Na2HPO4∗7 H2O
4.2 g KH2PO4
20 g agar
27.11.2018 - 19:30
After one week, the shape was different, it was more separated than when I cultivated them with Gluconcetobacter medium.
04.12.2018 - 19:30
In conclusion, Kombucha bacteria can be growing on both types of medium, with Gluconcetobacter medium they will form a shape like a colony, but with LBP medium they are more apart.
Growing Physarum polycephalum (2nd attempt)
I failed growing slime mold at my 1st attempt, so I tried once again with three dishes (just in case!), but this time I kept all of them inside the cabinet in the lab. I've learnt form my 1st attempt that my room is not a suitable environment for growing slime mold.
01.11.2018 - 10:30
06.11.2018 - 10:30 under the microscope
I moved them into another dishes, since the oats are completely covered by mold.
09.11.2018 - 13:30
After two weeks, they died (again) due to the unsuitable temperature. It's more difficult to grow the Physarum during winter.
Growing Bacteria from Chalk
I had Gluconcetobacter left, so I used that medium for growing bacteria from chalk. I expect to have some artworks form it, using a telescope camera.
30.10.2018 - 20:30
07.11.2018 - 10:30
They are contaminated, so I took one of the bacteria which is not fully contaminated to other dish.